Genome-wide analysis of flavonoid biosynthetic genes in Musaceae (Ensete, Musella, and Musa species) reveals amplification of flavonoid 3’,5’-hydroxylase

IF 2.6 3区 生物学 Q2 ECOLOGY
AoB Plants Pub Date : 2024-09-10 DOI:10.1093/aobpla/plae049
Dongli Cui, Gui Xiong, Lyuhan Ye, Richard Gornall, Ziwei Wang, Pat Heslop-Harrison, Qing Liu
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引用次数: 0

Abstract

Flavonoids in Musaceae are involved in pigmentation and stress responses, including cold resistance, and are a component of the healthy human diet. Identification and analysis of the sequence and copy number of flavonoid biosynthetic genes are valuable for understanding the nature and diversity of flavonoid evolution in Musaceae species. In this study, we identified 71 to 80 flavonoid biosynthetic genes in chromosome-scale genome sequence assemblies of Musaceae, including those of Ensete glaucum, Musella lasiocarpa, Musa beccarii, M. acuminata, M. balbisiana, and M. schizocarpa, checking annotations with BLAST and determining the presence of conserved domains. The number of genes increased through segmental duplication and tandem duplication. Orthologues of both structural and regulatory genes in the flavonoid biosynthetic pathway are highly conserved across Musaceae. The flavonoid 3’,5’-hydroxylase gene F3’5’H was amplified in Musaceae and ginger compared with grasses (rice, Brachypodium, Avena longiglumis, and sorghum). One group of genes from this gene family amplified near the centromere of chromosome 2 in the x = 11 Musaceae species. Flavonoid biosynthetic genes displayed few consistent responses in the yellow and red bracts of Musella lasiocarpa when subjected to low temperatures. The expression levels of MlDFR2/3 (dihydroflavonol reductase) increased while MlLAR (leucoanthocyanidin reductase) was reduced by half. Overall, the results establish the range of diversity in both sequence and copy number of flavonoid biosynthetic genes during evolution of Musaceae. The combination of allelic variants of genes, changes in their copy numbers, and variation in transcription factors with the modulation of expression under cold treatments and between genotypes with contrasting bract-colours suggests the variation may be exploited in plant breeding programmes, particularly for improvement of stress-resistance in the banana crop.
对麝香植物(Ensete、Musella 和 Musa 种)中黄酮类化合物生物合成基因的全基因组分析发现了黄酮类化合物 3',5'-羟化酶的扩增情况
麝香植物中的类黄酮参与色素沉着和抗寒等应激反应,是人类健康饮食的组成部分。鉴定和分析类黄酮生物合成基因的序列和拷贝数对于了解麝香植物类黄酮进化的性质和多样性很有价值。在这项研究中,我们在麝香科植物(包括Ensete glaucum、Musella lasiocarpa、Musa beccarii、M. acuminata、M. balbisiana和M. schizocarpa)的染色体级基因组序列组装中鉴定了71至80个黄酮类生物合成基因,用BLAST检查了注释并确定了保守结构域的存在。通过节段重复和串联重复,基因数量有所增加。黄酮类化合物生物合成途径中的结构基因和调控基因的同源物在麝香草科植物中高度保守。黄酮类化合物 3',5'-羟化酶基因 F3'5'H 在麝香草和生姜与禾本科植物(水稻、禾本科植物、莜麦和高粱)中都有扩增。在 x = 11 个麝香科物种中,该基因家族的一组基因在 2 号染色体中心点附近扩增。在低温条件下,麝香草黄苞片和红苞片中的黄酮类生物合成基因几乎没有一致的反应。MlDFR2/3(二氢黄酮醇还原酶)的表达水平有所增加,而MlLAR(白花青素还原酶)的表达水平则降低了一半。总之,研究结果确定了麝香草科植物进化过程中黄酮类生物合成基因序列和拷贝数的多样性范围。基因的等位基因变异、基因拷贝数的变化、转录因子的变化以及在冷处理条件下和苞片颜色对比强烈的基因型之间的表达调控等综合因素表明,这些变异可用于植物育种计划,特别是提高香蕉作物的抗逆性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
AoB Plants
AoB Plants PLANT SCIENCES-
CiteScore
4.80
自引率
0.00%
发文量
54
审稿时长
20 weeks
期刊介绍: AoB PLANTS is an open-access, online journal that has been publishing peer-reviewed articles since 2010, with an emphasis on all aspects of environmental and evolutionary plant biology. Published by Oxford University Press, this journal is dedicated to rapid publication of research articles, reviews, commentaries and short communications. The taxonomic scope of the journal spans the full gamut of vascular and non-vascular plants, as well as other taxa that impact these organisms. AoB PLANTS provides a fast-track pathway for publishing high-quality research in an open-access environment, where papers are available online to anyone, anywhere free of charge.
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