Biocompatibility and dimensional stability through the use of 3D‐printed scaffolds made by polycaprolactone and bioglass‐7: An in vitro and in vivo study

IF 3.7 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Ho‐Kyung Lim, In‐Seok Song, Won‐Cheul Choi, Young‐Jun Choi, Eun‐young Kim, Thi Hong Tham Phan, Ui‐Lyong Lee
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引用次数: 0

Abstract

PurposeThis experiment aimed to observe the differences in biological properties by producing BGS‐7 + PCL scaffolds with different weight fractions of BGS‐7 through 3D printing and to confirm whether using the scaffold for vertical bone augmentation is effective.Materials and MethodsCube‐shaped bioglass (BGS‐7) and polycaprolactone (PCL) scaffolds with different weight fractions (PCL alone, PCL with 15% and 30% BGS‐7) are produced using 3D printing. The surface hydroxyapatite (HA) apposition, the pH change, proliferation and attachment assays, and various gene expression levels are assessed. After a 7‐mm implant was inserted 3 mm into the rabbit calvaria, vertical bone augmentation is performed around the implant and inside the scaffold in four ways: scaffold only, scaffold+bone graft, bone graft only, and no graft. Sacrifice is performed at 6, 12, and 24 weeks, and the various parameters are compared radiographically and histologically.ResultsHA apposition, cell proliferation, cell attachment, and expression of osteogenic genes increase as the proportion of BGS‐7 increase. In the in vivo test, a higher bone–implant contact ratio, bone volume ratio, bone mineral density, and new bone area are observed when the scaffold and bone grafts were used together.ConclusionThe 3D‐printed scaffold, a mixture of BGS‐7 and PCL, exhibit higher biological compatibility as the proportion of BGS‐7 increase. Additionally, the use of scaffold is effective for vertical bone augmentation.
使用聚己内酯和生物玻璃-7 制成的 3D 打印支架的生物相容性和尺寸稳定性:体外和体内研究
本实验旨在通过三维打印技术制作不同重量分数的 BGS-7 + PCL 支架,观察其生物特性的差异,并确认使用该支架进行垂直骨增量是否有效。材料和方法使用三维打印技术制作不同重量分数的立方体生物玻璃(BGS-7)和聚己内酯(PCL)支架(单独 PCL、PCL 与 15% 和 30% BGS-7)。对表面羟基磷灰石(HA)附着、pH 值变化、增殖和附着试验以及各种基因表达水平进行了评估。将 7 毫米的植入物插入兔小腿 3 毫米后,在植入物周围和支架内部以四种方式进行垂直骨增量:仅支架、支架+骨移植、仅骨移植和无移植。结果随着 BGS-7 所占比例的增加,骨贴附、细胞增殖、细胞附着和成骨基因的表达也随之增加。在体内试验中,当支架和骨移植物一起使用时,可观察到更高的骨-植入物接触比、骨量比、骨矿物质密度和新骨面积。此外,使用支架还能有效增加垂直骨量。
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来源期刊
CiteScore
6.00
自引率
13.90%
发文量
103
审稿时长
4-8 weeks
期刊介绍: The goal of Clinical Implant Dentistry and Related Research is to advance the scientific and technical aspects relating to dental implants and related scientific subjects. Dissemination of new and evolving information related to dental implants and the related science is the primary goal of our journal. The range of topics covered by the journals will include but be not limited to: New scientific developments relating to bone Implant surfaces and their relationship to the surrounding tissues Computer aided implant designs Computer aided prosthetic designs Immediate implant loading Immediate implant placement Materials relating to bone induction and conduction New surgical methods relating to implant placement New materials and methods relating to implant restorations Methods for determining implant stability A primary focus of the journal is publication of evidenced based articles evaluating to new dental implants, techniques and multicenter studies evaluating these treatments. In addition basic science research relating to wound healing and osseointegration will be an important focus for the journal.
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