Skin derived branched-chain fatty acids in fetal horse gut

IF 1.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Dong Hao Wang, Lerong Qi, Tingxiang Yang, Yachen Ren, Zhen Wang
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引用次数: 0

Abstract

Branched-chain fatty acids (BCFAs) are mostly saturated fatty acids with a methyl branch at the penultimate or antepenultimate position to the CH3 end. Very-long-chain BCFAs (C ≥ 20) are uniquely found in surface lipids. Human and California sea lion (Zalophus californianus) are the only two species that possess vernix at birth, and vernix particles carry loads of BCFA to the gut, potentially influencing the establishment of the first microbiota. Horse (Equus caballus) contains high levels of BCFA in its surface lipids, and the current study aims to investigate if BCFA are components of a neonatal foal's gut. Electron ionization tandem mass spectrometry was used to distinguish iso- and anteiso-form of BCFA from straight-chain fatty acids. Abundant very-long-chain iso-BCFA, mainly even-numbered iso-20:0, iso-22:0, iso-24:0, and iso-26:0 were found in horse meconium but not in their manure collected a few months later. There are little differences in BCFA types and concentrations between foal manure and adult manure. Moderate to high levels of very-long-chain anteiso-BCFA were also found. Comparisons of horse meconium BCFA to horse skin cholesterol ester BCFA content and to meconium from human and sea lion suggest its skin derived origin. The study expands our knowledge on the gut nutritional environment of a newborn horse, and further study is needed to establish the relationship between gut BCFA and microbiota composition in the first few hours of life.

Practical Applications: Branched-chain fatty acids (BCFA) are bioactive compounds found in vernix caseosa, the lipid-laden material covering the surface of a newborn baby. BCFA also reaches high concentrations from the third trimester of gestation until birth. However, the exact functionality and mechanisms of BCFA in the surface and gut of a fetus remain unclear. The current study aims to clarify the lipid conditions in the gut of newborn foals and to identify the associations between BCFA in the gut and on the skin at a critical point when the horse's microbiota establishes itself. The findings of the current study lay a path for studying the physiological functions of BCFA in horse models, potentially accelerating research related to BCFA.

胎马肠道中源自皮肤的支链脂肪酸
支链脂肪酸(BCFA)大多是饱和脂肪酸,在 CH3 端倒数第二位或倒数第二位有一个甲基分支。超长链 BCFAs(C ≥ 20)独特地存在于表面脂质中。人类和加利福尼亚海狮(Zalophus californianus)是仅有的两个在出生时就拥有葡萄胎的物种,葡萄胎颗粒携带大量的 BCFA 进入肠道,可能会影响第一个微生物群的建立。马(Equus caballus)的表面脂质中含有大量的 BCFA,本研究旨在调查 BCFA 是否是新生马驹肠道中的成分。本研究采用电子电离串联质谱法从直链脂肪酸中区分出 BCFA 的异构和反异构形式。在马的胎粪中发现了丰富的超长链异BCFA,主要是偶数异-20:0、异-22:0、异-24:0和异-26:0,但在几个月后收集的马粪中却没有发现。马驹粪便和成年马粪中的 BCFA 类型和浓度差异不大。此外,还发现了中等至高含量的超长链前异BCFA。马粪中 BCFA 含量与马皮肤胆固醇酯 BCFA 含量以及人和海狮粪便中 BCFA 含量的比较表明,马粪中的 BCFA 来源于马皮肤。这项研究拓展了我们对新生马肠道营养环境的了解,我们还需要进一步研究,以确定马出生后几小时内肠道 BCFA 与微生物群组成之间的关系:支链脂肪酸(BCFA)是一种生物活性化合物,存在于覆盖在新生儿表面的含脂物质 "vernix caseosa "中。从妊娠三个月到出生,支链脂肪酸的浓度也很高。然而,BCFA 在胎儿表面和肠道中的确切功能和机制仍不清楚。本研究旨在阐明新生马驹肠道中的脂质状况,并在马匹微生物群建立的关键时刻,确定肠道中的 BCFA 与皮肤上的 BCFA 之间的关联。本研究的发现为在马模型中研究 BCFA 的生理功能铺平了道路,有可能加速 BCFA 的相关研究。
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来源期刊
CiteScore
5.50
自引率
0.00%
发文量
101
审稿时长
6-16 weeks
期刊介绍: The European Journal of Lipid Science and Technology is a peer-reviewed journal publishing original research articles, reviews, and other contributions on lipid related topics in food science and technology, biomedical science including clinical and pre-clinical research, nutrition, animal science, plant and microbial lipids, (bio)chemistry, oleochemistry, biotechnology, processing, physical chemistry, and analytics including lipidomics. A major focus of the journal is the synthesis of health related topics with applied aspects. Following is a selection of subject areas which are of special interest to EJLST: Animal and plant products for healthier foods including strategic feeding and transgenic crops Authentication and analysis of foods for ensuring food quality and safety Bioavailability of PUFA and other nutrients Dietary lipids and minor compounds, their specific roles in food products and in nutrition Food technology and processing for safer and healthier products Functional foods and nutraceuticals Lipidomics Lipid structuring and formulations Oleochemistry, lipid-derived polymers and biomaterials Processes using lipid-modifying enzymes The scope is not restricted to these areas. Submissions on topics at the interface of basic research and applications are strongly encouraged. The journal is the official organ the European Federation for the Science and Technology of Lipids (Euro Fed Lipid).
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