Nanoencapsulation of wheat germ oil with chitosan-nettle seed gum-ovalbumin: Preparation, optimization, and characterization

IF 1.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Gözde Kutlu, Perihan Kubra Akman, Kübra Feyza Erol, Fatih Bozkurt, Fatih Tornuk
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引用次数: 0

Abstract

This study was aimed to optimize nanoencapsulation conditions of ovalbumin (OV)-nettle seed gum (NSG)-chitosan (CS), ONC-based nanocarriers, using response surface methodology (RSM). Then, wheat germ oil (WGO) was encapsulated in optimized ONC nanocarriers, and WGO-loaded nanocarriers were characterized for their encapsulation efficiency, zeta potential, average particle size, emulsion stability, thermal, structural, micromorphological, rheological, and cytotoxic properties. The results showed that NSG-CS amount of 30 mL, OV amount of 20 mL, and pH of 3 gave the maximum zeta potential and minimum average particle size based on the predicted conditions for the production of ONC nanocarriers by RSM. Encapsulation efficiencies of WGO-loaded nanoparticles (WGONs) containing different ratios of WGO (2.5% to 4.5%, v:w) ranged from 48.66% to 71.91%. According to rheological properties, both the solutions of ONC complex and WGONs exhibited Newtonian flow behavior. The FT-IR spectra exhibited changes in the peaks associated with amino and carboxyl groups following the encapsulation of WGO, suggesting the successful completion of the encapsulation process. SEM images revealed that the addition of WGO into the capsule resulted in the formation of rough particles. The cytotoxicity tests demonstrated that concentrations of WGO, CS, NSG, OV, WGO, ONC, and WGON up to 10 mg mL–1 did not significantly reduce the viability of L-929 human fibroblast cell lines, indicating their nontoxicity toward healthy cells. In conclusion, this study suggested the successful encapsulation of WGO within ONC carriers at the nanoscale, achieving high encapsulation efficiency and favorable properties to enhance their uses for different purposes.

Practical Application: Wheat germ, an important by-product of wheat milling industry, contains considerable amounts of oil which possesses many beneficial components like tocopherol, vitamin B, phytosterols, and policosanols. These compounds have potential health benefits such as supporting exercise performance, cardiovascular health, and reducing the risk of overweight/obesity. Therefore, this underutilized oil needs to be introduced to the food/nutraceutical industry more extensively. However, the susceptibility of wheat germ oil (WGO) to oxidation and its short shelf-life limit its further use. Encapsulation technology can be employed to protect the WGO, extend its shelf-life, and improve its stability. Response surface methodology (RSM) is also a versatile way to determine the optimal conditions for encapsulation, resulting in the formation of stable nanocapsules containing WGO. The resulting nanocapsules effectively release the active ingredients of WGO, protecting them from oxidation and making them highly suitable for use as functional food ingredients or in pharmaceutical products with promising potential.

壳聚糖-菜籽胶-卵磷脂纳米封装小麦胚芽油:制备、优化和表征
本研究旨在利用响应面方法学(RSM)优化卵清蛋白(OV)-奈特尔种子胶(NSG)-壳聚糖(CS)(ONC基纳米载体)的纳米包囊条件。然后,将小麦胚芽油(WGO)封装在优化的 ONC 纳米载体中,并对负载 WGO 的纳米载体的封装效率、zeta 电位、平均粒径、乳液稳定性、热学、结构、微观形态、流变学和细胞毒性特性进行了表征。结果表明,根据 RSM 预测的 ONC 纳米载体生产条件,NSG-CS 用量为 30 mL,OV 用量为 20 mL,pH 值为 3 时,zeta 电位最大,平均粒径最小。不同WGO比例(2.5%至4.5%,v:w)的WGO负载纳米颗粒(WGONs)的封装效率为48.66%至71.91%。根据流变特性,ONC 复合物和 WGONs 的溶液都表现出牛顿流体特性。傅立叶变换红外光谱显示,在封装 WGO 后,与氨基和羧基相关的峰值发生了变化,表明封装过程已成功完成。扫描电镜图像显示,将 WGO 加入胶囊后形成了粗糙的颗粒。细胞毒性测试表明,浓度高达 10 mg mL-1 的 WGO、CS、NSG、OV、WGO、ONC 和 WGON 不会显著降低 L-929 人成纤维细胞系的活力,表明它们对健康细胞无毒性。总之,这项研究表明,在纳米尺度的 ONC 载体中成功封装了 WGO,实现了高封装效率和良好的性能,从而提高了它们在不同用途中的使用:小麦胚芽是小麦制粉工业的重要副产品,含有大量油脂,其中含有许多有益成分,如生育酚、维生素 B、植物甾醇和肌醇。这些化合物具有潜在的健康益处,如支持运动表现、心血管健康和降低超重/肥胖风险。因此,这种未得到充分利用的油类需要更广泛地引入食品/营养保健品行业。然而,小麦胚芽油(WGO)易氧化和货架期短的特点限制了它的进一步使用。封装技术可用于保护小麦胚芽油,延长其保质期并提高其稳定性。响应面法(RSM)也是确定最佳封装条件的一种通用方法,可形成含有 WGO 的稳定纳米胶囊。所形成的纳米胶囊能有效释放 WGO 的活性成分,保护其不被氧化,使其非常适合用作功能性食品配料或医药产品,具有广阔的应用前景。
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来源期刊
CiteScore
5.50
自引率
0.00%
发文量
101
审稿时长
6-16 weeks
期刊介绍: The European Journal of Lipid Science and Technology is a peer-reviewed journal publishing original research articles, reviews, and other contributions on lipid related topics in food science and technology, biomedical science including clinical and pre-clinical research, nutrition, animal science, plant and microbial lipids, (bio)chemistry, oleochemistry, biotechnology, processing, physical chemistry, and analytics including lipidomics. A major focus of the journal is the synthesis of health related topics with applied aspects. Following is a selection of subject areas which are of special interest to EJLST: Animal and plant products for healthier foods including strategic feeding and transgenic crops Authentication and analysis of foods for ensuring food quality and safety Bioavailability of PUFA and other nutrients Dietary lipids and minor compounds, their specific roles in food products and in nutrition Food technology and processing for safer and healthier products Functional foods and nutraceuticals Lipidomics Lipid structuring and formulations Oleochemistry, lipid-derived polymers and biomaterials Processes using lipid-modifying enzymes The scope is not restricted to these areas. Submissions on topics at the interface of basic research and applications are strongly encouraged. The journal is the official organ the European Federation for the Science and Technology of Lipids (Euro Fed Lipid).
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