IL-4, IL-7, IL-9, NT, NRP1 May Be Useful Markers in the Diagnosis of Endometrial Cancer

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomolecules Pub Date : 2024-09-01 DOI:10.3390/biom14091095

Mateusz Kozłowski, Dominika Borzyszkowska, Natalia Lerch, Agnieszka Turoń-Skrzypińska, Marta Tkacz, Jerzy Lubikowski, Maciej Tarnowski, Iwona Rotter, Aneta Cymbaluk-Płoska

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引用次数: 0

Abstract

The search for novel endometrial cancer diagnostic biomarkers is pertinent. The purpose of this study was to determine if IL-4, IL-7, IL-9, IL-10, NT, TSP-2, and NRP1 could be used as novel, helpful markers for the detection of endometrial cancer. Ninety-three women diagnosed with endometrial cancer (EC) and sixty-six patients with noncancerous endometrial lesions (NCEL) were included in this study. ELISA was used to measure the concentrations of the proteins tested. Median serum levels of IL-4, IL-7, IL-9, NT, and NRP1 were significantly higher in the EC group compared with NCEL. The cut-off level of IL-4 was set at 802.26 pg/mL with a sensitivity of 83.87% and a specificity of 50% (AUC = 0.7, p = 0.000023). The cut-off level of IL-7 was set at 133.63 ng/L with a sensitivity of 96.77% and a specificity of 75.76% (AUC = 0.91, p < 0.000001). The cut-off level of IL-9 was set at 228.79 pg/mL with a sensitivity of 69.89% and a specificity of 81.82% (AUC = 0.8, p < 0.000001). The cut-off level of NT was set at 275.43 pmol/L with a sensitivity of 94.62% and a specificity of 59.09% (AUC = 0.83, p < 0.000001). The cut-off level of NRP1 was set at 30.37 ng/mL with a sensitivity of 81.72% and a specificity of 57.58% (AUC = 0.71, p = 0.000004). This study suggests the clinical utility of IL-4, IL-7, IL-9, NT, and NRP1 in the diagnosis of endometrial cancer. Nevertheless, these biomarkers may also have prognostic or predictive value, which should be tested in future studies.

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IL-4、IL-7、IL-9、NT、NRP1 可能是诊断子宫内膜癌的有用标记物

寻找新型的子宫内膜癌诊断生物标志物具有现实意义。本研究的目的是确定IL-4、IL-7、IL-9、IL-10、NT、TSP-2和NRP1是否可用作检测子宫内膜癌的新型有用标记物。93名确诊为子宫内膜癌（EC）的妇女和66名非癌子宫内膜病变（NCEL）患者参与了这项研究。采用酶联免疫吸附法测定受测蛋白质的浓度。与NCEL相比，EC组患者血清中IL-4、IL-7、IL-9、NT和NRP1的中位水平明显较高。IL-4 的临界水平设定为 802.26 pg/mL，灵敏度为 83.87%，特异度为 50%（AUC = 0.7，p = 0.000023）。IL-7 的临界值设定为 133.63 ng/L，灵敏度为 96.77%，特异度为 75.76%（AUC = 0.91，p < 0.000001）。IL-9 的临界值设定为 228.79 pg/mL，灵敏度为 69.89%，特异度为 81.82%（AUC = 0.8，p < 0.000001）。NT的临界值为275.43 pmol/L，灵敏度为94.62%，特异度为59.09%（AUC = 0.83，p < 0.000001）。NRP1 的临界水平设定为 30.37 ng/mL，灵敏度为 81.72%，特异度为 57.58%（AUC = 0.71，p = 0.000004）。这项研究表明，IL-4、IL-7、IL-9、NT 和 NRP1 在诊断子宫内膜癌中具有临床实用性。不过，这些生物标志物也可能具有预后或预测价值，这需要在今后的研究中加以检验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.

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