Sample selection disparity: Sampling only cobble overestimates the biomass of stream benthic algae

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-09 DOI:10.1111/1440-1703.12523

Mitsuya Inoue, Kentaro Nozaki, Motomi Genkai‐Kato

{"title":"Sample selection disparity: Sampling only cobble overestimates the biomass of stream benthic algae","authors":"Mitsuya Inoue, Kentaro Nozaki, Motomi Genkai‐Kato","doi":"10.1111/1440-1703.12523","DOIUrl":null,"url":null,"abstract":"Despite the fact that scientists are aware that the streambed consists of various substrata in size, the estimation of benthic algal biomass has been conducted based almost exclusively on cobble sampling. This disparity in samples selected for the biomass estimation occurs because frame sampling collects all substrata, encompassed by the frame, including sand and stones, and is a time‐consuming method compared to single‐stone sampling. We conducted frame versus cobble sampling to test for sample selection disparity (SSD) in the estimation of benthic algal biomass. Estimates of algal biomass based on the frame sampling (area: 0.25 m2) were compared with those based on the cobble sampling taken at the same sampling points in a diatom‐dominated stream. Benthic algal biomass estimated based on cobble sampling was larger than the biomass estimated with frame sampling. The contribution of cobbles to the algal biomass encompassed by the frame was considerably higher than smaller substrata. These results suggest that cobble sampling tends to result in an overestimate of the benthic algal biomass in natural streams. Because the frame sampling requires intensive labor and time, we here proposed a general model based on quick visual assessments for percentage cover of cobbles on the streambed to calibrate estimates obtained by cobble sampling.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1111/1440-1703.12523","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Despite the fact that scientists are aware that the streambed consists of various substrata in size, the estimation of benthic algal biomass has been conducted based almost exclusively on cobble sampling. This disparity in samples selected for the biomass estimation occurs because frame sampling collects all substrata, encompassed by the frame, including sand and stones, and is a time‐consuming method compared to single‐stone sampling. We conducted frame versus cobble sampling to test for sample selection disparity (SSD) in the estimation of benthic algal biomass. Estimates of algal biomass based on the frame sampling (area: 0.25 m2) were compared with those based on the cobble sampling taken at the same sampling points in a diatom‐dominated stream. Benthic algal biomass estimated based on cobble sampling was larger than the biomass estimated with frame sampling. The contribution of cobbles to the algal biomass encompassed by the frame was considerably higher than smaller substrata. These results suggest that cobble sampling tends to result in an overestimate of the benthic algal biomass in natural streams. Because the frame sampling requires intensive labor and time, we here proposed a general model based on quick visual assessments for percentage cover of cobbles on the streambed to calibrate estimates obtained by cobble sampling.

查看原文本刊更多论文

样本选择差异：仅对卵石取样会高估溪流底栖藻类的生物量

尽管科学家们知道河床由大小不一的底层组成，但对底栖藻生物量的估算几乎都是在卵石取样的基础上进行的。之所以会出现这种生物量估算样本选择上的差异，是因为框架取样法收集了框架所涵盖的所有底层，包括沙子和石头，与单块石头取样法相比，框架取样法耗时较长。我们进行了框架取样与卵石取样对比，以检验底栖藻类生物量估算中的样本选择差异（SSD）。在以硅藻为主的溪流中，根据框架取样（面积：0.25 平方米）和在相同取样点进行卵石取样得出的藻类生物量估计值进行了比较。根据卵石取样估算的底栖藻类生物量大于框架取样估算的生物量。鹅卵石对框架取样所包含的藻类生物量的贡献要远远高于较小的底层。这些结果表明，卵石取样往往会高估自然溪流中的底栖藻类生物量。由于框架取样需要大量的人力和时间，我们在此提出了一个基于快速目测河床卵石覆盖率的通用模型，以校准卵石取样得到的估计值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.