Antiviral Activity of Angelica Tenuissima Nakai against Influenza A Virus

IF 3.3 3区医学 Q2 MICROBIOLOGY

Pathogens Pub Date : 2024-09-04 DOI:10.3390/pathogens13090761

Won-Jong Park, Gi-Sang Bae, Youn-Ho Han

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Abstract

The influenza A virus poses a serious threat to human health and is an important global public health issue. The drugs currently used for treatment are becoming increasingly ineffective against influenza A viruses and require the development of new antiviral drugs. Angelica tenuissima Nakai (ATN), a traditional herbal medicine belonging to the Umbelliferae family, exhibits a broad range of pharmacological activities, including inflammation, headache, and cold symptoms. In the present study, based on target protein identification, functional enrichment analysis, and gene set comparisons, we first suggested that ATN has potential therapeutic effects against influenza A virus infection. Next, methylthiazol tetrazolium (MTT) and sulforhodamine B colorimetric (SRB) assay results revealed that ATN exhibited low cytotoxicity in Madin–Darby canine kidney (MDCK) cells. The antiviral properties of ATN were observed against H1N1 and H3N2 virus strains. Microscopy confirmed the increased survival rate of the host cells. Further time-of-addition experiments revealed that the addition of ATN before virus adsorption showed similar results to the whole period of treatment. The pre- and co-treated groups showed lower levels of viral RNA (M1 protein). The results of this study suggest that ATN exhibits antiviral properties against the influenza A virus. These therapeutic properties of ATN can serve as a theoretical basis for further research on the applicability of ATN in the development of antiviral agents.

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中井当归对甲型流感病毒的抗病毒活性

甲型流感病毒严重威胁人类健康，是一个重要的全球公共卫生问题。目前用于治疗的药物对甲型流感病毒的疗效越来越差，因此需要开发新的抗病毒药物。当归（Angelica tenuissima Nakai，ATN）是一种伞形科传统草药，具有广泛的药理活性，包括炎症、头痛和感冒症状。在本研究中，基于靶蛋白鉴定、功能富集分析和基因组比较，我们首先提出 ATN 对甲型流感病毒感染具有潜在的治疗作用。接着，甲基噻唑四唑（MTT）和磺胺罗丹明 B 比色（SRB）检测结果显示，ATN 在马丁达比犬肾细胞（MDCK）中表现出较低的细胞毒性。ATN 对 H1N1 和 H3N2 病毒株具有抗病毒特性。显微镜检查证实，宿主细胞的存活率有所提高。进一步的添加时间实验显示，在病毒吸附前添加 ATN 与整个处理期间的结果相似。预处理组和联合处理组的病毒 RNA（M1 蛋白）水平较低。这项研究结果表明，ATN 具有抗甲型流感病毒的特性。ATN 的这些治疗特性可为进一步研究 ATN 在抗病毒药物开发中的适用性提供理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathogens Medicine-Immunology and Allergy

CiteScore

6.40

自引率

8.10%

发文量

1285

审稿时长

17.75 days

期刊介绍： Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.

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