Detection and characterization of the cowpea chlorotic mottle virus in forage peanut (Arachis pintoi) in Brazil

IF 0.9 4区农林科学 Q4 PLANT SCIENCES

Australasian Plant Pathology Pub Date : 2024-09-02 DOI:10.1007/s13313-024-00987-3

Késsia Fátima Cunha Pantoja, Alessandra de Jesus Boari, Bruno Rossitto De Marchi, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima, Rivadalve Coelho Gonçalves, Giselle Mariano Lessa Assis, Renate Krause-Sakate

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Abstract

The cowpea chlorotic mottle virus (CCMV) was detected in forage peanut plants (Arachis pintoi), showing mosaic, yellowing, and interveinal chlorosis. Negatively stained partially purified preparations contained isometric particles ca. 30 nm in size, also present in the cytoplasm and vacuole of leaf parenchymal cells from CCMV-infected A. pintoi leaves. Mechanical transmission of the CCMV leaf extracts from symptomatic A. pintoi plants resulted in systemic mosaic in A. pintoi cv. BRS Mandobi, Vigna unguiculata L. cv Pretinha, and V. unguiculata sg. sesquipedalis cv. De Metro plants; mottle on leaves of inoculated Nicotiana occidentalis and N. benthamiana; and local necrotic and chlorotic lesions in Chenopodium quinoa and C. amaranticolor, respectively. High-throughput sequencing (HTS) allowed us to identify the causal agent as the CCMV and obtain the complete nucleotide sequence of the three genome components. BLAST search in the nucleotide database revealed high identities of the genome components with previously reported CCMV isolates. Primer was designed based on the HTS-derived sequence and efficiently detected CCMV in the infected A. pintoi samples. To our knowledge, this is the first report of CCMV infecting forage peanuts.

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巴西牧草花生（Arachis pintoi）中豇豆萎黄斑驳病毒的检测和特征描述

在饲用花生植株（Arachis pintoi）中检测到了豇豆萎黄斑驳病毒（CCMV），该病毒表现为镶嵌、黄化和叶脉间萎黄。阴性染色的部分纯化制备物含有大小约为 30 nm 的等距颗粒，这些颗粒也存在于受 CCMV 感染的花生叶片实质细胞的细胞质和液泡中。从有症状的松果菊植株中提取的 CCMV 叶片提取物经机械传动后，会导致松果菊变种 BRS Mandobi、Vigna una、A. pintoi、A. pintoi、A. pintoi、A. pintoi cv.De Metro 植物的叶片上出现斑驳；接种的西洋烟（Nicotiana occidentalis）和 N. benthamiana 的叶片上出现斑驳；藜（Chenopodium quinoa）和 C. amaranticolor 的叶片分别出现局部坏死和萎黄病变。通过高通量测序（HTS），我们确定了病原体为 CCMV，并获得了三个基因组成分的完整核苷酸序列。核苷酸数据库中的 BLAST 搜索结果显示，这些基因组成分与之前报道的 CCMV 分离物具有很高的相同度。根据 HTS 序列设计的引物能有效检测出受感染的松鸡样本中的 CCMV。据我们所知，这是 CCMV 感染饲料花生的首次报道。

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来源期刊

Australasian Plant Pathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Australasian Plant Pathology presents new and significant research in all facets of the field of plant pathology. Dedicated to a worldwide readership, the journal focuses on research in the Australasian region, including Australia, New Zealand and Papua New Guinea, as well as the Indian, Pacific regions. Australasian Plant Pathology is the official journal of the Australasian Plant Pathology Society.