Exploring the Anti-Inflammatory and Antioxidative Potential of Selenium Nanoparticles Biosynthesized by Lactobacillus casei 393 on an Inflamed Caco-2 Cell Line

IF 1.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Azadeh Aghamohammadi Sendani, Maryam Farmani, Kasra Jahankhani, Nesa Kazemifard, Shaghayegh Baradaran Ghavami, Hamidreza Houri, Fatemeh Ashrafi, Amir Sadeghi
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引用次数: 0

Abstract

Selenium (Se) plays a crucial role in modulating inflammation and oxidative stress within the human system. Biogenic selenium nanoparticles (SeNPs) synthesized by Lactobacillus casei (L. casei) exhibit anti-inflammatory and anti-oxidative properties, positioning them as a promising alternative to traditional supplements characterized by limited bioavailability. With this context in mind, this study investigates the impact of selenium and L. casei in ameliorating inflammation and oxidative stress using a cell line model. The study is centered on the biosynthesis of selenium nanoparticles (SeNPs) by L. casei 393 under anaerobic conditions using a solution of sodium selenite (Na2SeO3) in the bacterial culture medium. The generation of SeNPs ensued from the interaction of L. casei bacteria with selenium ions, a process characterized via transmission electron microscopy (TEM) to confirm the synthesis of SeNPs. To induce inflammation, the human colonic adenocarcinoma cell line, Caco-2 was subjected to interleukin-1 beta (IL-1β) at concentrations of 0.5 and 25 ng/ml. Subsequent analyses encompass the evaluation of SeNPs derived from L. casei, its supernatant, commercial selenium, and L. casei probiotic on Caco2 cell line. Finally, we assessed the inflammatory and oxidative stress markers. The assessment of inflammation involved the quantification of NF-κB and TGF-β gene expression levels, while oxidative stress was evaluated through the measurement of Nrf2, Keap1, NOX1, and SOD2 gene levels. L. casei successfully produced SeNPs, as confirmed by the color change in the culture medium and TEM analysis showing their uniform distribution within the bacteria. In the inflamed Caco-2 cell line, the NF-κB gene was upregulated, but treatment with L. casei-SeNPs and selenium increased TGF-β expression. Moreover, L. casei-SeNPs upregulated SOD2 and Nrf2 genes, while downregulating NOX1, Keap1, and NF-κB genes. These results demonstrated the potential of L. casei-SeNPs for reducing inflammation and managing oxidative stress in the Caco-2 cell line. The study underscores the ability of L. casei-SeNPs to reduce oxidative stress and inflammation in inflamed Caco-2 cell lines, emphasizing the effectiveness of L. casei as a source of selenium. These insights hold significant promise for the development of SeNPs derived from L. casei as potent anti-inflammatory and anti-cancer agents, paving the way for novel therapeutic applications in the field.

Abstract Image

探索由干酪乳杆菌 393 生物合成的硒纳米颗粒在发炎的 Caco-2 细胞系上的抗炎和抗氧化潜力
硒(Se)在调节人体系统的炎症和氧化应激方面起着至关重要的作用。由干酪乳杆菌(L. casei)合成的生物硒纳米颗粒(SeNPs)具有抗炎和抗氧化特性,因此有望成为生物利用率有限的传统补充剂的替代品。有鉴于此,本研究利用细胞系模型研究了硒和干酪乳杆菌在改善炎症和氧化应激方面的影响。研究的核心是在厌氧条件下,利用细菌培养基中的亚硒酸钠(Na2SeO3)溶液,由酪酸杆菌 393 生物合成硒纳米颗粒(SeNPs)。SeNPs的产生源于干酪乳杆菌与硒离子的相互作用,这一过程通过透射电子显微镜(TEM)进行了表征,以确认SeNPs的合成。为了诱发炎症,人类结肠腺癌细胞系 Caco-2 接受了浓度为 0.5 和 25 纳克/毫升的白细胞介素-1β(IL-1β)。随后的分析包括评估从干酪乳杆菌、其上清液、商业硒和干酪乳杆菌益生菌中提取的 SeNPs 对 Caco2 细胞系的影响。最后,我们评估了炎症和氧化应激标记物。炎症评估涉及 NF-κB 和 TGF-β 基因表达水平的量化,而氧化应激则通过测量 Nrf2、Keap1、NOX1 和 SOD2 基因水平进行评估。培养基的颜色变化和显示细菌内部均匀分布的 TEM 分析证实,干酪乳杆菌成功地产生了 SeNPs。在发炎的 Caco-2 细胞系中,NF-κB 基因上调,但用 L. casei-SeNPs 和硒处理后,TGF-β 的表达增加。此外,L. casei-SeNPs 还能上调 SOD2 和 Nrf2 基因,同时下调 NOX1、Keap1 和 NF-κB 基因。这些结果证明了 L. casei-SeNPs 在 Caco-2 细胞系中减少炎症和管理氧化应激的潜力。该研究强调了干酪乳杆菌-SeNPs 在发炎的 Caco-2 细胞系中减少氧化应激和炎症的能力,强调了干酪乳杆菌作为硒源的有效性。这些见解为开发从干酪乳杆菌中提取的 SeNPs 作为强效抗炎剂和抗癌剂带来了重大希望,为该领域的新型治疗应用铺平了道路。
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来源期刊
Cell Biochemistry and Biophysics
Cell Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
4.40
自引率
0.00%
发文量
72
审稿时长
7.5 months
期刊介绍: Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.
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