The optimization of antioxidant and anti-inflammatory activities of carotenoids, total polyphenols, and indole alkaloids from coral Astroides calycularis and their interactions using simplex-centroid designs

IF 2.2 4区化学 Q2 Engineering

Chemical Papers Pub Date : 2024-08-17 DOI:10.1007/s11696-024-03643-8

Meriem Adouane, Nabil Kadri, Nourelimane Benzitoune, Chafika Lakhdari, Samia Djellal, Lilla Ousmer, Ourdia-Nouara Kernou, Hocine Remini, Farid Dahmoune, Khodir Madani

{"title":"The optimization of antioxidant and anti-inflammatory activities of carotenoids, total polyphenols, and indole alkaloids from coral Astroides calycularis and their interactions using simplex-centroid designs","authors":"Meriem Adouane, Nabil Kadri, Nourelimane Benzitoune, Chafika Lakhdari, Samia Djellal, Lilla Ousmer, Ourdia-Nouara Kernou, Hocine Remini, Farid Dahmoune, Khodir Madani","doi":"10.1007/s11696-024-03643-8","DOIUrl":null,"url":null,"abstract":"<div><p>This research aimed to examine the interplay among carotenoids, total polyphenols, and indole alkaloids for the discovery of new bioactive compounds using “simplex-centroid” designs and response surface methodology (RSM). The antioxidant (AOA) and anti-inflammatory (AIA) activities of mixtures containing different proportions of each compound were evaluated by measuring the percentage inhibition of oxidation and spontaneous inflammation. The results showed that the highest AOAs were obtained with different mixtures of polyphenols, indole alkaloids, and carotenoids. The DPPH test revealed that a mixture of 16.94% polyphenols and 83.05% indole alkaloids produced the best results, whereas, for the ABTS test, 100% polyphenols gave the best results. The FRAP assay showed that the best-performing mixture was composed of 85.96% carotenoids and 14.03% polyphenols, with an IC<sub>50</sub> of 8.85 ± 1.17 μg/mL. In contrast, the most potent AIA was obtained with 100% carotenoids. Model validation tests were performed and showed that the experimentally observed values for AOA and AIA were not significantly different from the values estimated by the fitted models. The results also showed that the RSM model can be used to estimate the behavior of mixed compounds in the development of new functional ingredients or the discovery of new bioactive compounds with pharmacological properties. Finally, the results also suggested that there is a good correlation between several AOA and AIA assays, which makes natural substances combining these biological activities particularly interesting.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":513,"journal":{"name":"Chemical Papers","volume":"78 14","pages":"7905 - 7925"},"PeriodicalIF":2.2000,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Papers","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s11696-024-03643-8","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}

引用次数: 0

Abstract

This research aimed to examine the interplay among carotenoids, total polyphenols, and indole alkaloids for the discovery of new bioactive compounds using “simplex-centroid” designs and response surface methodology (RSM). The antioxidant (AOA) and anti-inflammatory (AIA) activities of mixtures containing different proportions of each compound were evaluated by measuring the percentage inhibition of oxidation and spontaneous inflammation. The results showed that the highest AOAs were obtained with different mixtures of polyphenols, indole alkaloids, and carotenoids. The DPPH test revealed that a mixture of 16.94% polyphenols and 83.05% indole alkaloids produced the best results, whereas, for the ABTS test, 100% polyphenols gave the best results. The FRAP assay showed that the best-performing mixture was composed of 85.96% carotenoids and 14.03% polyphenols, with an IC₅₀ of 8.85 ± 1.17 μg/mL. In contrast, the most potent AIA was obtained with 100% carotenoids. Model validation tests were performed and showed that the experimentally observed values for AOA and AIA were not significantly different from the values estimated by the fitted models. The results also showed that the RSM model can be used to estimate the behavior of mixed compounds in the development of new functional ingredients or the discovery of new bioactive compounds with pharmacological properties. Finally, the results also suggested that there is a good correlation between several AOA and AIA assays, which makes natural substances combining these biological activities particularly interesting.

Graphical abstract

Abstract Image

查看原文本刊更多论文

使用简单中心设计优化珊瑚中类胡萝卜素、总多酚和吲哚生物碱的抗氧化和抗炎活性及其相互作用

这项研究的目的是利用 "简单中心 "设计和响应面方法（RSM）研究类胡萝卜素、总多酚和吲哚生物碱之间的相互作用，以发现新的生物活性化合物。通过测量氧化和自发炎症的抑制百分比，评估了含有不同比例的每种化合物的混合物的抗氧化（AOA）和抗炎（AIA）活性。结果表明，多酚、吲哚生物碱和类胡萝卜素的不同混合物的 AOA 值最高。DPPH 试验表明，16.94% 的多酚和 83.05% 的吲哚生物碱混合物产生的结果最好，而 ABTS 试验中，100% 的多酚产生的结果最好。FRAP 试验表明，表现最好的混合物由 85.96% 的类胡萝卜素和 14.03% 的多酚组成，IC50 为 8.85 ± 1.17 μg/mL。相比之下，100% 类胡萝卜素的 AIA 效果最好。进行的模型验证测试表明，实验观察到的 AOA 和 AIA 值与拟合模型估计值没有显著差异。结果还表明，在开发新的功能性成分或发现具有药理特性的新生物活性化合物时，RSM 模型可用于估计混合化合物的行为。最后，研究结果还表明，几种 AOA 和 AIA 检测方法之间存在很好的相关性，这使得结合了这些生物活性的天然物质变得特别有趣。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemical Papers Chemical Engineering-General Chemical Engineering

CiteScore

3.30

自引率

4.50%

发文量

590

期刊介绍： Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.