Monitoring of patulin and ochratoxin A in apple juices using covalent organic framework-reinforced hollow fiber-solid phase microextraction method and HPLC

IF 2.2 4区 化学 Q2 Engineering
Ali Sadeghi Alavian, Ali Mohebbi, Saeid Yaripour, Mir Ali Farajzadeh
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Abstract

In the current work, a covalent organic framework-reinforced hollow fiber-solid phase microextraction method has been introduced for the extraction of patulin and ochratoxin A from commercial apple juices before their determination with high-performance liquid chromatography-tandem mass spectrometry. This method benefited from the advantages of traditional hollow fiber-liquid phase microextraction and solid phase microextraction methods. Briefly, in this method, after preparation of the covalent organic framework-reinforced hollow fiber through a simple method, it was placed into the sample solution and the mixture was stirred to increase the contact surface. After stopping the stirring process, the hollow fiber was taken and placed into a vial containing choline chloride: dichloroacetic acid deep eutectic solvent as an elution solvent. In the following, the mixture was sonicated to accelerate the desorption process. Finally, the eluate containing the extracted analytes was taken and injected into separation system. Under optimized conditions, validation of the developed method was done and the outcomes indicated great precision (relative standard deviations ≤ 5.2%), good linearity (coefficient of determination ≥ 0.9974) in a wide range (77.3–1 × 106 ng L−1), and low limits of detection (23.2 and 15.6 ng L−1 for patulin and ochratoxin A, respectively) and quantification (77.3 and 52.1 ng L−1 for patulin and ochratoxin A, respectively). At the end, the suggested approach was employed for the analysis of patulin and ochratoxin A in thirty commercial apple juice samples. Based on the results, patulin and ochratoxin A were detected in 17% and 23% of the investigated apple juice samples, respectively.

Abstract Image

利用共价有机框架增强型中空纤维固相微萃取法和高效液相色谱法监测苹果汁中的棒曲霉素和赭曲霉毒素 A
本研究介绍了一种共价有机框架增强中空纤维固相微萃取方法,用于从商品苹果汁中萃取棒曲霉素和赭曲霉毒素 A,然后用高效液相色谱-串联质谱法进行测定。该方法借鉴了传统的中空纤维液相微萃取法和固相微萃取法的优点。简而言之,该方法通过简单的方法制备出共价有机框架增强中空纤维后,将其放入样品溶液中,搅拌混合物以增加接触面。停止搅拌后,将中空纤维放入装有氯化胆碱:二氯乙酸深度共晶溶剂的小瓶中作为洗脱溶剂。随后,对混合物进行超声处理,以加速解吸过程。最后,取含有提取分析物的洗脱液注入分离系统。在优化的条件下,对所建立的方法进行了验证,结果表明该方法精密度高(相对标准偏差小于 5.2%),线性关系好(测定系数≥ 0.9974),检出限(棒曲霉素和赭曲霉毒素 A 的检出限分别为 23.2 和 15.6 ng L-1)和定量限(棒曲霉素和赭曲霉毒素 A 的定量限分别为 77.3 和 52.1 ng L-1)均较宽。最后,我们采用建议的方法分析了 30 个商业苹果汁样本中的棒曲霉素和赭曲霉毒素 A。结果显示,在调查的苹果汁样本中,分别有 17% 和 23% 检测到棒曲霉素和赭曲霉毒素 A。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemical Papers
Chemical Papers Chemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍: Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.
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