Evaluation of cytotoxic potential of silver nanoparticles biosynthesized using essential oils of Jasminum sambac against breast cancer and bacterial cells

IF 2.6 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2024-09-10 DOI:10.1007/s13205-024-04058-8

Ali Kadhum Bidan, Zainab Shakir Abdullah Al-Ali

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Abstract

Essential oils (EOs) which cover about 91% whole biomolecules formulated from Jasminum sambac leaves based on Gas chromatography-mass spectrometry were employed to identify structures. EOs were observed as good agents in the preparation of Silver nanoparticles (AgNPs) through the proposed mechanism that was attempted to interpret the pathway of the bio-preparation process. The characterization of EOs-AgNPs carried via ultraviolet–visible to reveal surface plasmon resonance at 420 nm, Fourier transform infrared to observe functional groups EOs compared to EOs-AgNPs. X-ray diffraction (XRD) revealed a broad chart owing to the small size of AgNPs in average size less than 10 nm calculated relying on image J software, spherical AgNPs with a small dispersive size observed by transmission electron microscopy. Quasi near spherical surface morphology of EOs-AgNPs had detected by field emission scanning electron microscope. EOs-AgNPs were assessed for their antibacterial potential against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria as suppressing bacterial agents. EOs-AgNPs had their anti-breast cancer MCF-7 cell line ability investigated by DNA fragmentation; cycle flow cytometry (apoptosis) at half maximal inhibitory concentration (IC₅₀) was determined at 260 µg/mL which has been stated by cytotoxicity (MTT) assay. EOs-AgNPs have antibacterial and anticancer therapeutic potential, and it is safe, inexpensive, and scalable in the nanoscale range.

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评估使用茉莉精油生物合成的银纳米粒子对乳腺癌细胞和细菌细胞的细胞毒性潜力

根据气相色谱-质谱分析法，从茉莉叶中提取的精油（EOs）涵盖了整个生物大分子的约 91%，用于确定其结构。通过尝试解释生物制备过程的路径所提出的机制，观察到环氧乙烷是制备银纳米粒子（AgNPs）的良好制剂。EOs-AgNPs 的表征是通过紫外可见光来揭示 420 纳米波长的表面等离子共振，通过傅立叶变换红外来观察 EOs 与 EOs-AgNPs 的功能基团。X 射线衍射（XRD）显示了一个宽广的图表，这是由于 AgNPs 的尺寸较小，根据图像 J 软件计算得出的平均尺寸小于 10 nm，透射电子显微镜观察到的球形 AgNPs 散射尺寸较小。场发射扫描电子显微镜检测到 EOs-AgNPs 接近球形的表面形态。评估了环氧乙烷-AgNPs 作为抑菌剂对革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌）的抗菌潜力。环氧乙烷-AgNPs 抗乳腺癌 MCF-7 细胞系的能力是通过 DNA 断裂法、循环流式细胞术（细胞凋亡）和细胞毒性（MTT）测定法确定的，半数最大抑制浓度（IC50）为 260 µg/mL。环氧乙烷-AgNPs 具有抗菌和抗癌治疗潜力，而且在纳米级范围内安全、廉价、可扩展。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.

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