The complete mitochondrial genomes of three Evacanthini species, Evacanthus bivittatus, Carinata ganga, and Carinata recurvata, and phylogenomic analysis of the Evacanthini

IF 2.4 3区环境科学与生态学 Q2 ECOLOGY

Frontiers in Ecology and Evolution Pub Date : 2024-08-29 DOI:10.3389/fevo.2024.1410546

Sai Jiang, Ran Li, Lina Jiang, Wei Wang, Yongcheng Liu, Yuewei Yang, Jichun Xing, Zizhong Li, Yujian Li

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Abstract

The mitochondrial genome is recognized for its utility in insect molecular research, due to its distinctive features, including fast evolutionary rate, low recombination, and maternal inheritance. In this study, we explored the mitochondrial genome in the context of the leafhopper subfamily Evacanthinae, which is significant in the agricultural pest sector due to the direct and indirect damage caused to plants by its species. We present complete mitochondrial genome sequences for three species: Evacanthus bivittatus, Carinata ganga, and Carinata recurvata. Comparative analyses of nucleotide composition, codon usage of protein coding genes (PCGs), nucleotide diversity, transfer RNA secondary structure, and gene overlap were conducted. To understand phylogenetic relationships among species, we constructed phylogenetic trees using nucleotide sequences from the 13 PCGs of the genomes newly sequenced in this study alongside other available leafhopper genome sequences. Phylogenetic analysis supported monophyly of the Evacanthinae subfamily and suggested a close relationship between the Evacanthini and Nirvanini tribes. Our research indicates that anticodon differences in trnS1 are insufficient to serve as taxonomic markers for distinguishing between these two tribes. This study contributes valuable genetic data supporting ongoing investigations into genetic diversity, molecular evolution, and species identification, while laying groundwork for future taxonomic and evolutionary endeavors within Cicadellidae.

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Evacanthus bivittatus、Carinata ganga 和 Carinata recurvata 这三个 Evacanthini 物种的完整线粒体基因组以及 Evacanthini 的系统发生组分析

线粒体基因组因其进化速度快、重组率低和母系遗传等显著特点而被公认为是昆虫分子研究的有用工具。在本研究中，我们以叶蝉亚科（Evacanthinae）为背景，对线粒体基因组进行了探索。叶蝉亚科在农业害虫领域具有重要地位，因为其物种对植物造成了直接和间接的损害。我们展示了三个物种的完整线粒体基因组序列：Evacanthus bivittatus、Carinata ganga 和 Carinata recurvata。我们对核苷酸组成、蛋白质编码基因（PCGs）的密码子使用、核苷酸多样性、转移核糖核酸二级结构和基因重叠进行了比较分析。为了解物种间的系统发育关系，我们利用本研究中新测序的基因组中 13 个 PCGs 的核苷酸序列与其他可用的叶蝉基因组序列一起构建了系统发生树。系统发育分析支持Evacanthinae亚科的单系性，并表明Evacanthini和Nirvanini部落之间关系密切。我们的研究表明，trnS1的反密码子差异不足以作为区分这两个部落的分类标记。这项研究为正在进行的遗传多样性、分子进化和物种鉴定研究提供了宝贵的遗传数据支持，同时也为今后蝉科内的分类和进化工作奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Ecology and Evolution Environmental Science-Ecology

CiteScore

4.00

自引率

6.70%

发文量

1143

审稿时长

12 weeks

期刊介绍： Frontiers in Ecology and Evolution publishes rigorously peer-reviewed research across fundamental and applied sciences, to provide ecological and evolutionary insights into our natural and anthropogenic world, and how it should best be managed. Field Chief Editor Mark A. Elgar at the University of Melbourne is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics and the public worldwide. Eminent biologist and theist Theodosius Dobzhansky’s astute observation that “Nothing in biology makes sense except in the light of evolution” has arguably even broader relevance now than when it was first penned in The American Biology Teacher in 1973. One could similarly argue that not much in evolution makes sense without recourse to ecological concepts: understanding diversity — from microbial adaptations to species assemblages — requires insights from both ecological and evolutionary disciplines. Nowadays, technological developments from other fields allow us to address unprecedented ecological and evolutionary questions of astonishing detail, impressive breadth and compelling inference. The specialty sections of Frontiers in Ecology and Evolution will publish, under a single platform, contemporary, rigorous research, reviews, opinions, and commentaries that cover the spectrum of ecological and evolutionary inquiry, both fundamental and applied. Articles are peer-reviewed according to the Frontiers review guidelines, which evaluate manuscripts on objective editorial criteria. Through this unique, Frontiers platform for open-access publishing and research networking, Frontiers in Ecology and Evolution aims to provide colleagues and the broader community with ecological and evolutionary insights into our natural and anthropogenic world, and how it might best be managed.