Selection of DNA aptamers for sensing drugs treating eye disease: atropine and timolol maleate†‡

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Ka-Ying Wong, Yibo Liu, Chau-Minh Phan, Lyndon Jones, Man-Sau Wong and Juewen Liu
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引用次数: 0

Abstract

Effective monitoring of ocular drugs is crucial for personalized medicine and improving drug delivery efficacy. However, traditional methods face difficulties in detecting low drug concentrations in small volumes of ocular fluid, such as that found on the ocular surface. In this study, we used capture-SELEX to select aptamers for two commonly used ocular drugs, timolol maleate and atropine. We identified TMJ-1 and AT-1 aptamers with binding affinities of 3.4 μM timolol maleate and 10 μM atropine, respectively. Our label-free TMJ-1 biosensor using thioflavin T staining achieved a limit of detection (LOD) of 0.3 μM for timolol maleate. The AT-1 biosensor showed an LOD of 1 μM for atropine, and exhibited a 10-fold higher sensitivity compared to UV-visible spectroscopy. Future research in this area holds promise in enhancing drug delivery monitoring and improving the treatment of ocular diseases.

Abstract Image

Abstract Image

选择用于感知治疗眼疾药物的 DNA 合体:阿托品和马来酸噻吗洛尔
有效监测眼部药物对于个性化医疗和提高给药效果至关重要。然而,传统方法难以检测小体积眼液中的低药物浓度,如眼表面的药物浓度。在这项研究中,我们使用捕获-SELEX来选择两种常用眼部药物(马来酸噻吗洛尔和阿托品)的适配体。我们鉴定出的 TMJ-1 和 AT-1 合体的结合亲和力分别为 3.4 μM 马来酸噻吗洛尔和 10 μM 阿托品。我们使用硫黄素 T 染色的无标记 TMJ-1 生物传感器对马来酸噻吗洛尔的检测限(LOD)为 0.3 μM。AT-1 生物传感器对阿托品的检测限为 1 μM,灵敏度比紫外可见光谱法高 10 倍。该领域的未来研究有望加强给药监测并改善眼部疾病的治疗。
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CiteScore
2.30
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