Development and Validation of a High-Performance Thin-Layer Chromatography Method for Simultaneous Qualitative and Quantitative Analysis of Scutellarin and Scutellarein in S. barbata D. Don

IF 0.7 4区医学 Q4 PHARMACOLOGY & PHARMACY

Current Pharmaceutical Analysis Pub Date : 2024-08-15 DOI:10.2174/0115734129312130240812070549

Arzoo Pannu, Ramesh K. Goyal

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引用次数: 0

Abstract

Background: S. barbata D. Don, a perennial herb from the Lamiaceae family, is renowned for its medicinal properties, with scutellarin and scutellarein being key bioactive constituents. Aim: In this study, we present the development and validation of a High-Performance Thin- Layer Chromatography (HPTLC) method for the simultaneous quantitative and qualitative analysis of scutellarin and scutellarein in the hydroethanol extract of S. barbata D. Don. Methods: The chromatographic conditions were optimized using different solvent systems, and validation was performed as per ICH guidelines. Results: The mobile phase comprising Ethyl acetate: methanol: formic acid: water (20: 2.7: 0.5: 2) and scanning wavelength set at 254 nm was optimized for study, resulting in distinct and well-separated spots for scutellarin and scutellarein with Rf values of 0.31 and 0.96, respectively. The method was rigorously validated to ensure reliability and reproducibility. Calibration curves exhibited excellent linearity for both scutellarin (r² > 0.98) and scutellarein (r² > 0.99) over a concentration range of 10-30 μg/ml. The developed method underwent thorough validation following ICH guidelines. Validation parameters included accuracy, limit of quantification, limit of detection, linearity, precision, and recovery. The determined concentration of scutellarin and scutellarein in the hydroethanolic extract of S. barbata D. Don was found to be 27.00 μg/mg and 20.16 μg/mg of extract, respectively. Conclusion: This proposed method demonstrates its utility for both qualitative and quantitative analyses of scutellarin and scutellarein in the hydroethanolic extract of S. barbata D. Don, offering a reliable and validated approach for quality control and standardization in the herbal product industry.

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用于同时定性和定量分析 S. barbata D. Don 中黄芩苷和黄芩素的高效薄层色谱法的开发与验证

背景：S. barbata D. Don 是一种唇形科多年生草本植物，以其药用特性而闻名，其中黄芩苷和黄芩萃取物是其主要的生物活性成分。目的：本研究开发并验证了一种高效薄层色谱（HPTLC）方法，用于同时定量和定性分析豨莶草水乙醇提取物中的黄芩苷和黄芩萃取物。方法：使用不同的溶剂系统优化色谱条件，并根据 ICH 指南进行验证。结果：经优化的流动相为乙酸乙酯：甲醇：甲酸：水（20：2.7：0.5：2），扫描波长为 254 nm，结果表明黄芩苷和黄芩萃取物的色谱图清晰、分离度高，Rf 值分别为 0.31 和 0.96。该方法经过了严格的验证，以确保其可靠性和重现性。在10-30 μg/ml的浓度范围内，黄芩苷和黄芩素的线性关系良好（r² > 0.98）。所开发的方法按照 ICH 指南进行了全面的验证。验证参数包括准确度、定量限、检测限、线性、精密度和回收率。结果表明，在 S. barbata D. Don 的水乙醇提取物中，黄芩苷和黄芩萃取物的测定浓度分别为 27.00 μg/mg 和 20.16 μg/mg。结论所提出的方法证明了它在定性和定量分析巴豆水乙醇提取物中的黄芩苷和黄芩萃取物方面的实用性，为草药产品行业的质量控制和标准化提供了一种可靠、有效的方法。

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来源期刊

Current Pharmaceutical Analysis 医学-药学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Aims & Scope Current Pharmaceutical Analysis publishes expert reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.