Prevalence of Wolbachia infection in field natural population of the apricot seed wasp Eurytoma samsonowi (Hymenoptera: Eurytomidae)

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-12 DOI:10.1007/s10123-024-00593-9

Takwa Wannassi, Ahmed Sayadi, Khaled Abbes, Salma Djebbi, Chahnez Naccache, Maha Mezghani Khemakhem, Brahim Chermiti

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Abstract

Obligate endosymbiont bacteria associated with insects are naturally providing their hosts with essential nutrients such as vitamins and amino acids and biological services including protection from pathogens. In this study, we aimed to investigate the presence of Wolbachia infection among males and females of the parasitic apricot seed wasp (ASW) Eurytoma samsonowi Vassiliev (Vassiliev Petrograd 11: 1-15, 1915) (Hymenoptera: Eurytomidae), a very harmful pest of apricot (Prunus armeniaca), in the oasis of Gafsa, Southern-West of Tunisia. The detection of Wolbachia infection was assessed based on the amplification of the Wolbachia surface protein (wsp) gene and a multilocus sequence typing (MLST) as a universal genotyping tool for Wolbachia involving the analyses of genes gatB, coxA, hcpA, fbpA, and ftsz. Confirming the screening results, Wolbachia was detected in the natural apricot wasp for the first time, with a significant difference between males (5%) and females (59%) based on wsp gene. All Wolbachia strains identified in E. samsonowi were clustered among supergroups B of Wolbachia.

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杏核蜂（膜翅目：Eurytomidae）野外自然种群中沃尔巴克氏体的感染率

与昆虫相关的内生细菌可自然地为宿主提供必需的营养物质（如维生素和氨基酸）和生物服务，包括保护宿主免受病原体的侵害。在这项研究中，我们的目的是调查突尼斯西南部加夫萨绿洲的寄生杏核蜂（ASW）Eurytoma samsonowi Vassiliev（Vassiliev Petrograd 11: 1-15, 1915）（膜翅目：Eurytomidae）（一种对杏（Prunus armeniaca）非常有害的害虫）的雄蜂和雌蜂中是否存在沃尔巴克氏体感染。根据沃尔巴克氏体表面蛋白（wsp）基因的扩增和作为沃尔巴克氏体通用基因分型工具的多焦点序列分型（MLST）（包括分析 gatB、coxA、hcpA、fbpA 和 ftsz 基因），对沃尔巴克氏体感染的检测进行了评估。根据 wsp 基因，在雄性（5%）和雌性（59%）之间存在显著差异。在杏小蜂中发现的所有沃尔巴克氏体菌株都属于沃尔巴克氏体B超群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464