Disposable glutamate biosensor based on platinum nanoparticles, carbon quantum dots and poly-L-aspartic acid.

IF 2 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-12 DOI:10.1080/10826068.2024.2402340

Ceren Kaçar Selvi,Barış Şenceol,Pınar Esra Erden

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Abstract

This study reports the design and development of a disposable amperometric biosensor for the determination of L-glutamate. Glutamate oxidase (GlOx) was immobilized onto a screen-printed carbon electrode (SPE) modified with poly-L-Aspartic acid (PAsp), carbon quantum dots (CQD), and platinum nanoparticles (PtNP) for the construction of the biosensor. The surface composition of the modified SPE was optimized using the one variable at a time method. The morphological properties of the biosensor were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The electrochemical behavior of the modified electrodes was studied by cyclic voltammetry. Under the optimized experimental conditions the linear working range, detection limit and sensitivity of the GlOx/PtNP/CQD/PAsp/SPE were found to be 1.0 - 140 µM, 0.3 µM and 0.002 µA µM-1, respectively. The GlOx/PtNP/CQD/PAsp/SPE biosensor also exhibited good measurement repeatability. The as-developed biosensor was applied for the determination of L-glutamate in spiked serum samples and the average analytical recovery of added glutamate was 98.9 ± 3.9%.

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基于铂纳米粒子、碳量子点和聚天冬氨酸的一次性谷氨酸生物传感器。

本研究报告了一种用于测定 L-谷氨酸的一次性安培生物传感器的设计与开发。在构建该生物传感器时，将谷氨酸氧化酶（GlOx）固定在用聚天冬氨酸（PAsp）、碳量子点（CQD）和铂纳米颗粒（PtNP）修饰的丝网印刷碳电极（SPE）上。采用一次一变量法优化了改性 SPE 的表面成分。利用扫描电子显微镜和能量色散 X 射线光谱对生物传感器的形态特性进行了表征。通过循环伏安法研究了改性电极的电化学行为。在优化的实验条件下，GlOx/PtNP/CQD/PAsp/SPE 的线性工作范围、检测限和灵敏度分别为 1.0 - 140 µM、0.3 µM 和 0.002 µA µM-1。GlOx/PtNP/CQD/PAsp/SPE 生物传感器还表现出良好的测量重复性。将所开发的生物传感器用于测定添加了谷氨酸的血清样品中的 L-谷氨酸，添加谷氨酸的平均分析回收率为 98.9 ± 3.9%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Preparative Biochemistry & Biotechnology 工程技术-生化研究方法

CiteScore

4.90

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.