Spectral Properties of Biodyes in the Formation of New Preparative Forms of Prodrugs

Abstract

The spectral and bactericidal properties of a number of biodyes including bromophenol blue, amido black 10B, methyl thymol blue, basic fuchsin, chrome dark blue, eosin, indigo dye, and bromophenol red have been characterized. The inclusion of eosin, bromophenol red, indigo, and rifampicin into the internal space of phospholipid nanocontainers was shown to lead to leveling of the characteristic absorption maxima in the visible and ultraviolet regions of the spectra of these compounds taken in aqueous solution. This effect allows visual and spectrophotometric monitoring of the prodrug incorporation into liposomes. The construction of a number of supramolecular forms of prodrugs, which are micelles and liposomes derived from modifications of the bactericidal nucleoside brivudine in various combinations, showed the feasibility of using indigo as a dye marker. This permits the identification of liposomes formed from an equimolar mixture of dimyristoylphosphatidylbrivudine lipoconjugate with dimyristoylphosphatidylcholine as a potential prodrug formulation that most effectively inhibits the growth of Staphylococcus aureus cells.