Construction of ferroptosis-related gene signatures for identifying potential biomarkers and immune cell infiltration in osteoarthritis.

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-09-11 DOI:10.1080/21691401.2024.2402298

Yali Yu,Guixiang Dong,Yanli Niu

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引用次数: 0

Abstract

Osteoarthritis (OA) is a comprehensive joint disorder. The specific genes that trigger OA and the strategies for its effective management are not fully understood. This study focuses on identifying key genes linked to iron metabolism that could influence both the diagnosis and therapeutic approaches for OA. Analysis of GEO microarray data and iron metabolism genes identified 15 ferroptosis-related DEGs, enriched in hypoxia and HIF-1 pathways. Ten key hub genes (ATM, GCLC, PSEN1, CYBB, ATG7, MAP1LC3B, PLIN2, GRN, APOC1, SIAH2) were identified. Through stepwise regression, we screened 4 out of the above 10 genes, namely, GCLC, GRN, APOC1, and SIAH2, to obtain the optimal model. AUROCs for diagnosis of OA for the four hub genes were 0.81 and 0.80 of training and validation sets, separately. According to immune infiltration results, OA was related to significantly increased memory B cells, M0 macrophages, regulatory T cells, and resting mast cells but decreased activated dendritic cells. The four hub genes showed a close relation to them. It is anticipated that this model will aid in diagnosing osteoarthritis by assessing the expression of specific genes in blood samples. Moreover, studying these hub genes may further elucidate the pathogenesis of osteoarthritis.

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构建铁蛋白沉积相关基因特征，以确定骨关节炎的潜在生物标志物和免疫细胞浸润。

骨关节炎（OA）是一种综合性关节疾病。引发 OA 的特定基因以及有效治疗 OA 的策略尚未完全明了。本研究的重点是确定与铁代谢有关的关键基因，这些基因可能会影响 OA 的诊断和治疗方法。对 GEO 微阵列数据和铁代谢基因的分析发现了 15 个与铁代谢相关的 DEGs，这些 DEGs 富集于缺氧和 HIF-1 通路。确定了10个关键枢纽基因（ATM、GCLC、PSEN1、CYBB、ATG7、MAP1LC3B、PLIN2、GRN、APOC1、SIAH2）。通过逐步回归，我们筛选出了上述 10 个基因中的 4 个，即 GCLC、GRN、APOC1 和 SIAH2，从而获得了最佳模型。在训练集和验证集中，这4个中心基因诊断OA的AUROC分别为0.81和0.80。免疫浸润结果显示，OA 与记忆性 B 细胞、M0 巨噬细胞、调节性 T 细胞和静止肥大细胞的显著增加有关，但与活化树突状细胞的减少有关。四个枢纽基因显示出与它们的密切关系。通过评估血液样本中特定基因的表达，预计该模型将有助于诊断骨关节炎。此外，研究这些枢纽基因可能会进一步阐明骨关节炎的发病机制。

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.