Microbacterium rhizophilus sp. nov., an indole acetic acid-producing actinobacterium isolated from rhizosphere soil

IF 1.8 3区生物学 Q4 MICROBIOLOGY

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-13 DOI:10.1007/s10482-024-02014-3

Haifei Liu, Quan Yang, Jiawei Li, Lifang Yang, Aolin Zhao, Ying Huang, Hongcun Liu, Shujing Wu, Mingguo Jiang

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Abstract

A novel gram-stain-positive, short rod, aerobic, non-motile and non-spore-forming actinobacterial strain, designated GXG1230^T was isolated from the rhizosphere soil of a coastal mangrove forest in Beihai city, Guangxi Zhuang Autonomous Region, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXG1230^T was affiliated with the genus Microbacterium. Additionally, it demonstrated a high degree of similarity to Microbacterium paludicola US15^T (97.9%) and Microbacterium marinilacus YM11-607^T (97.3%). Chemotaxonomic characteristics showed that the whole-cell sugars were glucose, xylose, rhamnose and galactose. Menaquinones MK-11 and MK-12 were detected as respiratory quinones. Lysine was found in the peptidoglycan hydrolysate and the polar lipids were diphosphatidylglycerol, one phospholipid and two unidentified glycolipid. The major fatty acids were anteiso-C_15:0, iso-C_16:0 and anteiso-C_17:0. The strain GXG1230^T exhibited a genomic DNA G + C content of 71.7%. Furthermore, the average nucleotide identity values of GXG1230^T with the reference strains were 75.4% and 81.9%, respectively, while the digital DNA-DNA hybridization values were 20.1% and 25.0%. Based on physiological, chemotaxonomic and phylogenetic information, strain GXG1230^T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium rhizophilus sp.nov is proposed, with GXG1230^T (= MCCC 1K09302^T = KCTC 59252^T) as the type strain.

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从根瘤土壤中分离出的产吲哚乙酸放线菌--根瘤嗜水微菌新种

研究人员从中国广西壮族自治区北海市沿海红树林根瘤土壤中分离到一株革兰氏染色阳性、短杆、好氧、无运动性、无芽孢的新型放线菌，命名为 GXG1230T。基于 16S rRNA 基因序列的系统进化分析表明，菌株 GXG1230T 属于微杆菌属。此外，GXG1230T与Microbacterium paludicola US15T（97.9%）和Microbacterium marinilacus YM11-607T（97.3%）具有高度相似性。化学分类学特征显示，全细胞糖类为葡萄糖、木糖、鼠李糖和半乳糖。检测到了作为呼吸醌的门醌 MK-11 和 MK-12。在肽聚糖水解物中发现了赖氨酸，极性脂质为二磷脂酰甘油、一种磷脂和两种不明糖脂。主要脂肪酸为前异-C15:0、异-C16:0 和前异-C17:0。菌株 GXG1230T 的基因组 DNA G + C 含量为 71.7%。此外，GXG1230T 与参考菌株的平均核苷酸同一性值分别为 75.4% 和 81.9%，数字 DNA-DNA 杂交值分别为 20.1% 和 25.0%。根据生理、化学分类学和系统发育信息，认为 GXG1230T 菌株代表了微杆菌属的一个新种，并将其命名为根瘤微杆菌 sp.nov，GXG1230T（= MCCC 1K09302T = KCTC 59252T）为模式菌株。

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来源期刊

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 生物-微生物学

CiteScore

5.60

自引率

11.50%

发文量

104

审稿时长

3 months

期刊介绍： Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.