L-cysteine capped MoS2 QDs for dual-channel imaging and superior Fe3+ ion sensing in biological systems

IF 4.6 3区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Nanoscale Advances Pub Date : 2024-09-13 DOI:10.1039/d4na00505h

Vishakha Takhar, Simranjit Singh, Superb Misra, Rupak Banerjee

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Abstract

MoS2 quantum dots (MQDs) with an average size of 1.9±0.7 nm were synthesized using a microwave-assisted method. Absorbance studies confirmed characteristic transitions of MoS2, with absorption humps at 260-280 nm and 300-330 nm, and a band gap of 3.6 ± 0.1 eV. Fluorescence emission studies showed dominant blue and some green emissions under 315 nm excitation, with an absolute quantum yield of ~ 9%. The MQDs exhibited fluorescence stability over time after repeated quenching cycles across various pH and media systems. In vitro toxicity tests indicated cytocompatibility, with around 80% cell survival at 1000 mg L-1. Confocal imaging demonstrated significant uptake and vibrant fluorescence in cancerous and non-cancerous cell lines. The MQDs showed strong selectivity towards Fe3+ ions, with a detection limit of 27.61 ± 0.25 nM. Recovery rates for Fe3+ in phosphate buffer saline (PBS) and simulated body fluid (SBF) systems were >97% and >98%, respectively, with a relative standard deviation (RSD) within 3%, indicating precision. These findings suggest that MQDs have high potential for diagnostic applications involving Fe3+ detection due to their fluorescence stability, robustness, enhanced cell viability, and dual-channel imaging properties.

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用于生物系统中双通道成像和卓越 Fe3+ 离子传感的 L-半胱氨酸封端 MoS2 QD

利用微波辅助方法合成了平均尺寸为 1.9±0.7 nm 的 MoS2 量子点（MQDs）。吸光研究证实了 MoS2 的特征转变，在 260-280 纳米和 300-330 纳米处有吸收驼峰，带隙为 3.6±0.1 eV。荧光发射研究显示，在 315 纳米激发下，主要发出蓝色，也有一些发出绿色，绝对量子产率约为 9%。在不同的 pH 值和介质体系中，MQDs 在反复淬灭循环后表现出长期的荧光稳定性。体外毒性测试表明，MQDs 具有细胞相容性，在 1000 mg L-1 的条件下，细胞存活率约为 80%。共焦成像显示，癌细胞和非癌细胞系对 MQDs 有明显的吸收，且荧光活跃。MQDs 对 Fe3+ 离子具有很强的选择性，检测限为 27.61 ± 0.25 nM。在磷酸盐缓冲盐水（PBS）和模拟体液（SBF）系统中，Fe3+的回收率分别为97%和98%，相对标准偏差（RSD）在3%以内，表明了其精确性。这些研究结果表明，MQDs 因其荧光稳定性、鲁棒性、增强的细胞活力和双通道成像特性，在涉及 Fe3+ 检测的诊断应用中具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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