Two functionally interchangeable Vps9 isoforms mediate pollen tube penetration of style

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-09-11 DOI:10.1111/nph.20088

Guang-Jiu Hao, Jun Ying, Lu-Shen Li, Fei Yu, Shan-Shan Dun, Le-Yan Su, Xin-Ying Zhao, Sha Li, Yan Zhang

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引用次数: 0

Abstract

Style penetration by pollen tubes is essential for reproductive success, a process requiring canonical Rab5s in Arabidopsis. However, functional loss of Arabidopsis Vps9a, the gene encoding for guanine nucleotide exchange factor (GEF) of Rab5s, did not affect male transmission, implying the presence of a compensation program or redundancy.
By combining genetic, cytological, and molecular approaches, we report that Arabidopsis Vps9b is a pollen-preferential gene, redundantly mediating pollen tube penetration of style with Vps9a.
Vps9b is functionally interchangeable with Vps9a, whose functional distinction results from distinct expression profiles.
Functional loss of Vps9a and Vps9b results in the mis-targeting of Rab5-dependent tonoplast proteins, defective vacuolar biogenesis, disturbed distribution of post-Golgi vesicles, increased cellular turgor, cytosolic acidification, and disrupted organization of actin microfilaments (MF) in pollen tubes, which collectively lead to the failure of pollen tubes to grow through style.

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两种功能可互换的 Vps9 同工酶介导花粉管穿透花柱

摘要拟南芥花粉管的花柱穿透对繁殖成功至关重要，这一过程需要典型的 Rab5s。然而，拟南芥 Vps9a（Rab5s 的鸟嘌呤核苷酸交换因子（GEF）的编码基因）的功能缺失并不影响雄性传递，这意味着存在补偿程序或冗余。通过结合遗传学、细胞学和分子方法，我们报告了拟南芥 Vps9b 是一个花粉偏爱基因，与 Vps9a 重叠介导花粉管穿透花柱。Vps9b 与 Vps9a 在功能上可以互换，其功能区别来自于不同的表达谱。Vps9a 和 Vps9b 的功能缺失会导致花粉管中依赖 Rab5 的调质蛋白靶向错误、液泡生物生成缺陷、高尔基体后囊泡分布紊乱、细胞张力增加、细胞酸化以及肌动蛋白微丝（MF）组织紊乱，这些因素共同导致花粉管无法穿透花柱生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.