SmJAZs-SmbHLH37/SmERF73-SmSAP4 module mediates jasmonic acid signaling to balance biosynthesis of medicinal metabolites and salt tolerance in Salvia miltiorrhiza

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-09-12 DOI:10.1111/nph.20110

Bingbing Lv, Huaiyu Deng, Jia Wei, Qiaoqiao Feng, Bo Liu, Anqi Zuo, Yichen Bai, Jingying Liu, Juane Dong, Pengda Ma

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引用次数: 0

Abstract

Salvia miltiorrhiza holds significant importance in traditional Chinese medicine. Stress-associated proteins (SAP), identified by A20/AN1 zinc finger structural domains, play crucial roles in regulating plant growth, development, resistance to biotic and abiotic stress, and hormone responses.
Herein, we conducted a genome-wide identification of the SAP gene family in S. miltiorrhiza. The expression analysis revealed a significant upregulation of SmSAP4 under methyl jasmonate (MeJA) and salt stress. Overexpressing SmSAP4 in S. miltiorrhiza hairy roots increased tanshinones content while decreasing salvianolic acids content, while RNAi-silencing SmSAP4 had the opposite effect. SmSAP4 overexpression in both Arabidopsis thaliana and S. miltiorrhiza hairy roots decreased their salt stress tolerance, accompanied by increased activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and a hindered ability to maintain the Na⁺ : K⁺ ratio.
Further investigations demonstrated that MeJA alleviated the inhibitory effect of SmJAZ3 on SmSAP4 activation by SmbHLH37 and SmERF73. However, MeJA did not affect the inhibition of SmSAP4 activation by SmJAZ8 through SmbHLH37.
In summary, our research reveals that SmSAP4 negatively regulates the accumulation of salvianic acid through the SmJAZs-SmbHLH37/SmERF73-SmSAP4 module and positively impacting the accumulation of tanshinones. Additionally, it functions as a negative regulator under salt stress.

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SmJAZs-SmbHLH37/SmERF73-SmSAP4模块介导茉莉酸信号，平衡丹参药用代谢物的生物合成和耐盐性

摘要丹参在传统中药中具有重要地位。由 A20/AN1 锌指结构域鉴定的胁迫相关蛋白（SAP）在调节植物生长、发育、抗生物和非生物胁迫以及激素反应方面发挥着重要作用。在此，我们对 S. miltiorrhiza 中的 SAP 基因家族进行了全基因组鉴定。表达分析表明，在茉莉酸甲酯（MeJA）和盐胁迫下，SmSAP4的表达明显上调。在S. miltiorrhiza毛根中过表达SmSAP4会增加丹参酮的含量，同时降低丹参酚酸的含量，而RNAi沉默SmSAP4则会产生相反的效果。在拟南芥和根瘤菌毛根中过表达 SmSAP4 会降低它们对盐胁迫的耐受性，同时会增加超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性，并阻碍维持 Na+ : K+ 比例的能力。进一步的研究表明，MeJA减轻了SmJAZ3对SmbHLH37和SmERF73激活SmSAP4的抑制作用。然而，MeJA 并不影响 SmJAZ8 通过 SmbHLH37 对 SmSAP4 激活的抑制作用。综上所述，我们的研究发现，SmSAP4 通过 SmJAZs-SmbHLH37/SmERF73-SmSAP4 模块负向调节丹参酸的积累，正向影响丹参酮的积累。此外，它还是盐胁迫下的负调控因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.