Functional impacts of PtrMYB203 on phenylpropanoid pathway regulation and wood properties in hybrid poplar

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-09-12 DOI:10.1016/j.plaphy.2024.109118

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Abstract

The phenylpropanoid pathway is vital for plant growth and development, producing lignin and flavonoids. This study investigates PtrMYB203, a homolog of MYB repressors of proanthocyanidin (PA) biosynthesis in Populus trichocarpa, as a transcriptional repressor in the phenylpropanoid pathway of hybrid poplar (Populus alba x P. glandulosa). Overexpression of PtrMYB203 (35S::PtrMYB203) in hybrid poplar detrimentally impacted plant growth and development. Histological analysis revealed irregular xylem vessel formation and decreased lignin content, corroborated by Klason lignin assays. Moreover, 35S::PtrMYB203 transgenic poplars exhibited significant decreases in anthocyanin and PA accumulations in callus tissues, even under high light conditions. Quantitative RT-PCR analysis and protoplast-based transcriptional activation assay confirmed the downregulation of lignin and flavonoid biosynthesis genes. This genetic modification also alters the expression of several MYB transcription factors, essential for phenylpropanoid pathway regulation. Remarkably, saccharification efficiency in the 35S::PtrMYB203 poplar was improved by over 34% following hot water treatment alone. These findings suggest PtrMYB203 as a potential genetic target for enhancing wood properties for bioenergy production, providing valuable insights into the manipulation of metabolite pathways in woody perennials to advance wood biotechnology.

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PtrMYB203 对杂交杨树苯丙酮途径调控和木材特性的功能影响

苯丙酮途径对植物的生长和发育至关重要，它能产生木质素和类黄酮。本研究调查了 PtrMYB203，它是杨树原花青素（PA）生物合成的 MYB 抑制因子的同源物，是杂交杨树（白杨 x P. glandulosa）苯丙酮途径中的转录抑制因子。在杂交杨中过表达 PtrMYB203（35S::PtrMYB203）会对植物的生长和发育产生不利影响。组织学分析表明，木质部血管形成不规则，木质素含量降低，克拉森木质素测定也证实了这一点。此外，即使在强光条件下，35S::PtrMYB203 转基因杨树胼胝体组织中的花青素和 PA 积累也显著减少。定量 RT-PCR 分析和基于原生质体的转录激活分析证实了木质素和类黄酮生物合成基因的下调。这种基因修饰还改变了几个 MYB 转录因子的表达，这些转录因子对苯丙氨酸途径的调控至关重要。值得注意的是，仅在热水处理后，35S::PtrMYB203 杨树的糖化效率就提高了 34% 以上。这些研究结果表明，PtrMYB203 是提高生物能源生产中木材特性的潜在遗传靶标，为操纵多年生木本植物的代谢途径以推动木材生物技术的发展提供了宝贵的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.