Molecular and morphological analysis revealed a new Lipoptena species (Diptera: Hippoboscidae) in southern Spain harbouring Coxiella burnetii and bacterial endosymbionts

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2024-09-03 DOI:10.1016/j.vetpar.2024.110300

Mikel Alexander González , Ignacio Ruiz-Arrondo , Sergio Magallanes , Jozef Oboňa , María José Ruiz-López , Jordi Figuerola

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引用次数: 0

Abstract

Hippoboscid flies (Diptera: Hippoboscidae) are obligate bloodsucking ectoparasites of animals. In Europe, limited research has been conducted on this family until the recent introduction of the deer ked Lipoptena fortisetosa Maa, 1965. A new species of the genus Lipoptena, Lipoptena andaluciensis sp. nov., was found in southern Spain after extensive sampling with carbon-dioxide baited suction traps. A total of 52 females and 32 males were collected at 29 out of 476 sites examined over eight months in 2023. Lipoptena andaluciensis sp. nov. was characterized morphologically and molecularly. The new Lipoptena species can be differentiated from the closely related L. fortisetosa by size, chaetotaxy of the dorsal and ventral thorax, abdominal plates, and genitalia. Based on DNA-barcoding, our specimens showed the highest similarity with Melophagus ovinus (Linnaeus, 1758) (88.4 %) and with L. fortisetosa (86–88 %). Individual screening of Lipoptena specimens (n = 76) for seven important zoonotic pathogens such as bacteria (Anaplasmataceae family: Bartonella spp., Borrelia spp., Coxiella burnetii and Rickettsia spp.) and protozoans (Babesia spp. and Theileria spp.) by conventional PCR and RT-PCR was performed. DNA of C. burnetii was detected in one specimen, while two other specimens harboured Anaplasmataceae (Wolbachia spp., 100 % homology and another endosymbiont probably related to Arsenophonus sp., 95.3 % homology, respectively), all representing the first records of these bacteria in the Lipoptena spp. from Europe. Carbon dioxide traps probed its effectiveness as a reliable passive method for keds surveillance. Our study highlights the existence of a new Lipoptena species, presumably widely distributed in southern Spain. The role of this species in the transmission cycle of pathogens of medical-veterinary relevance needs to be considered in the area.

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分子和形态分析揭示了西班牙南部的一个新 Lipoptena 种（双翅目：Hippoboscidae），该种携带烧伤柯西氏菌和细菌内生体

河马蝇（双翅目：Hippoboscidae）是动物的强制性吸血外寄生虫。在欧洲，对该科的研究十分有限，直到最近引入了鹿科蝇属的 Lipoptena fortisetosa Maa（1965 年）。在使用二氧化碳诱饵吸捕器进行广泛采样后，在西班牙南部发现了 Lipoptena 属的一个新物种 Lipoptena andaluciensis sp.在 2023 年的 8 个月中，在 476 个考察点中的 29 个考察点共采集到 52 只雌性和 32 只雄性。对 Lipoptena andaluciensis sp.该 Lipoptena 新种与近缘的 L. fortisetosa 的区别在于体型、胸部背面和腹部的序列、腹板和生殖器。根据 DNA 条形码，我们的标本与 Melophagus ovinus (Linnaeus, 1758) 的相似度最高（88.4 %），与 L. fortisetosa 的相似度最低（86-88 %）。对 Lipoptena 标本（n = 76）进行了 7 种重要人畜共患病病原体的单独筛查，如细菌（Anaplasmataceae 家族、Bartonella spp：通过传统 PCR 和 RT-PCR 方法对细菌（无形体科：巴氏杆菌属、包柔氏菌属、烧伤科克西氏菌属和立克次体属）和原生动物（巴贝斯虫属和泰勒虫属）进行了筛查。在一个标本中检测到了烧伤蜱的 DNA，而另外两个标本则携带有 Anaplasmataceae（Wolbachia spp.，同源性为 100%，另一种内生菌可能与 Arsenophonus sp.有关，同源性分别为 95.3%），这些都是欧洲首次在唇形目动物中发现这些细菌。二氧化碳捕集器探测了其作为一种可靠的被动方法对 Keds 进行监测的有效性。我们的研究强调了一种可能广泛分布于西班牙南部的 Lipoptena 新物种的存在。该地区需要考虑该物种在医疗兽医相关病原体传播循环中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.