Genome-wide identification and expression analysis of CmoADHs in Cucurbita moschata—Critical role of CmoADH9 in drought tolerance

Abstract

In plants, alcohol dehydrogenases (ADHs) are involved in stress response, organ development, fruit ripening, and metabolite synthesis. However, little is known regarding ADH-encoding genes (ADHs) in Cucurbita moschata which is usually used as a rootstock for cucumber, melon, watermelon, and other cucurbit crops to resist soil-borne diseases and abiotic stresses. We identified 11 CmoADHs in the C. moschata genome that were unevenly distributed across seven chromosomes. These genes were predicted to encode stable cytoplasmic acidic proteins, sharing a low degree of identity with each other. The genes exhibited different intron–exon structures. Analysis of cis-acting regulatory elements showed that CmoADHs contain environmental stress-, hormone response-, light response-, and development/tissue specificity-related elements in their promoters. Expression pattern analysis revealed that CmoADH2, CmoADH3, CmoADH4, CmoADH9, CmoADH10, and CmoADH11 had the highest expression levels in the roots, which were significantly higher than those in the other tested tissues. These six genes may play important roles in the growth and development of roots, and in related abiotic stress responses. CmoADH1, CmoADH5, CmoADH6, CmoADH7, CmoADH8 had the highest expression in the apical region and could be involved in the differentiation of newly formed tissues. To study the role of CmoADHs in abiotic stress, salt, drought, low temperature, and ethephon treatments were performed. Under drought conditions, CmoADHs showed different expression trends. The expression levels of CmoADH1, CmoADH2, CmoADH3, and CmoADH9 increased significantly and peaked after 1 h of drought treatment, indicating that these four genes are more sensitive to drought stress. Under salt treatment, all CmoADHs showed a significant increase or decrease in expression within 6 h, except for CmoADH5 and CmoADH10, which were insensitive to salt treatment. The expression of most of the CmoADHs was significantly downregulated by low-temperature treatment. Ethephon treatment significantly induced the expression of all the CmoADHs, except CmoADH2, to different degrees within 12 h. CmoADH9 was found to be involved in root growth and drought stress resistance. Identification of these ADH genes can provide useful resources for conferring stress resistance in other economically important crops.