Tracking the geographical distribution of the Asian citrus psyllid Diaphorina citri throughout China using mitogenomes and endosymbionts

IF 4.3 1区 农林科学 Q1 ENTOMOLOGY
Fengnian Wu, Zehan Dai, Min Shi, Jianjian Huang, Hui Zhu, Yuzhong Zheng, Zikai Chen, Xiuhong Li, Xiaoling Deng, Eduardo G. P. Fox
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Abstract

Diaphorina citri is the vector of “Candidatus Liberibacter asiaticus” (CLas), a bacterium associated with the citrus disease known as Huanglongbing (HLB). Previous mitochondrial genome (i.e. mitogenome) population analyses revealed the prevalence of two major mitochondrial groups (MGs) of D. citri in China, separated by elevation gradients. We assessed the population diversity of D. citri from 54 major citrus-producing areas within 11 provinces/regions of China. Additionally, endosymbiont genomes were assembled for “Ca. Carsonella ruddii” (CaCr) and “Ca. Profftella armatura” (CaPa) from next-generation sequencing of 31 new Chinese samples. Most of the D. citri diversity came from single nucleotide polymorphisms (SNPs) within five mitochondrial genes: nad3, cox2, rrnL, cob, and atp6. Nine SNPs clustered the analyzed D. citri mitogenomes into three major MGs comprising seven subgroups. Independent phylogenetic trees were generated for the endosymbionts CaCr and CaPa, and a CaPa plasmid, supporting the patterns obtained for D. citri mitogenomes while adding complexity layers. Genomic data from CaCr, CaPa, along with the plasmids from CaPa contribute to the genetic diversity—consisting of 68 SNPs and two genomic gaps—reproducing phylogenetic structures outlined by previous mitochondrial genomic studies. Additionally, both the mitogenomes and the endosymbiont genomes revealed subgroups within the original MG clusters, based on further 154 SNPs and 17 gaps. Thus, the combined genomic approach reveals further aspects about population diversity and natural history of this invasive species. Further understanding of D. citri and its endosymbionts can, therefore, aid D. citri HLB management protocols and help forecast territorial expansion events.

Abstract Image

利用有丝分裂基因组和内生共生体追踪亚洲柑橘木虱在中国各地的地理分布情况
枸橘褐飞虱是 "亚洲自由杆菌"(CLas)的病媒,这种细菌与柑橘黄龙病(HLB)有关。之前的线粒体基因组(即有丝分裂基因组)种群分析显示,中国的枸橘褐飞虱有两个主要的线粒体群(MGs),它们被海拔梯度分隔开来。我们评估了中国 11 个省区 54 个柑橘主产区的柑橘褐飞虱种群多样性。此外,我们还为 "Ca.Carsonella ruddii"(CaCr)和 "Ca.此外,通过对 31 个中国新样本进行下一代测序,为 "Ca. Carsonella ruddii"(CaCr)和 "Profftella armatura"(CaPa)组装了内生菌基因组。柠檬蝇的多样性主要来自于五个线粒体基因中的单核苷酸多态性(SNPs):NAD3、Cox2、RRNL、COB和ATP6。九个 SNPs 将分析的柠檬蝇有丝分裂基因组聚类为三个主要的 MGs,包括七个亚群。为内共生体 CaCr 和 CaPa 以及 CaPa 质粒生成了独立的系统发生树,在增加复杂性的同时,也支持了 D. citri 有丝分裂基因组所获得的模式。来自 CaCr、CaPa 和 CaPa 质粒的基因组数据为遗传多样性做出了贡献--包括 68 个 SNP 和两个基因组缺口--再现了之前线粒体基因组研究所勾勒的系统发育结构。此外,有丝分裂基因组和内共生体基因组还根据另外 154 个 SNP 和 17 个缺口,揭示了原始 MG 群组中的亚群。因此,综合基因组学方法揭示了这一入侵物种种群多样性和自然历史的更多方面。因此,进一步了解枸橘褐斑病及其内共生菌有助于制定枸橘褐斑病 HLB 管理方案,并帮助预测领土扩张事件。
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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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