Pad Printing of Carbon Electrodes with Argon Plasma Activation as a Simple and Low Temperature Manufacturing Process for Antibody-Type Biosensors

Enrico Condemi, Joanna Kunikowski, Spyridon Schoinas, Philippe Passeraub
{"title":"Pad Printing of Carbon Electrodes with Argon Plasma Activation as a Simple and Low Temperature Manufacturing Process for Antibody-Type Biosensors","authors":"Enrico Condemi,&nbsp;Joanna Kunikowski,&nbsp;Spyridon Schoinas,&nbsp;Philippe Passeraub","doi":"10.1002/adsr.202400015","DOIUrl":null,"url":null,"abstract":"<p>In diagnostic tools, rapid in vitro tests such as COVID-19 antigen or pregnancy tests are gaining significance for identifying various pathologies or health conditions. This shift contributes to a change in the way diagnostic efforts are carried out, emphasizing decentralized approaches that offer valuable services within communities, yielding long-term advantages for the healthcare system. Considering the substantial quantity of these tests manufactured and used annually, a straightforward manufacturing process is proposed for highly sensitive carbon electrodes designed for antibody-type biomarker sensors. This process, utilizing pad printing – an additive, low-temperature, and cost-effective method, coupled with plasma activation – has proven the electrodes capability to measure interferon gamma protein, a tuberculosis biomarker. Using electrochemical impedance spectroscopy, the electrodes display high sensitivity and are capable of measuring concentrations from 10 to 1000 pg mL<sup>−1</sup> in undiluted serum within an hour. The sensor, utilizing solely a monolayer of antibodies, achieves a performance equivalent to that of a commercial standard sandwich ELISA tested in this study.</p>","PeriodicalId":100037,"journal":{"name":"Advanced Sensor Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adsr.202400015","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Sensor Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/adsr.202400015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

In diagnostic tools, rapid in vitro tests such as COVID-19 antigen or pregnancy tests are gaining significance for identifying various pathologies or health conditions. This shift contributes to a change in the way diagnostic efforts are carried out, emphasizing decentralized approaches that offer valuable services within communities, yielding long-term advantages for the healthcare system. Considering the substantial quantity of these tests manufactured and used annually, a straightforward manufacturing process is proposed for highly sensitive carbon electrodes designed for antibody-type biomarker sensors. This process, utilizing pad printing – an additive, low-temperature, and cost-effective method, coupled with plasma activation – has proven the electrodes capability to measure interferon gamma protein, a tuberculosis biomarker. Using electrochemical impedance spectroscopy, the electrodes display high sensitivity and are capable of measuring concentrations from 10 to 1000 pg mL−1 in undiluted serum within an hour. The sensor, utilizing solely a monolayer of antibodies, achieves a performance equivalent to that of a commercial standard sandwich ELISA tested in this study.

Abstract Image

氩等离子活化碳电极的移印技术,作为抗体型生物传感器的一种简单低温制造工艺
在诊断工具方面,COVID-19 抗原或妊娠测试等快速体外测试在确定各种病症或健康状况方面的重要性日益凸显。这种转变有助于改变诊断工作的方式,强调在社区内提供有价值服务的分散方法,从而为医疗保健系统带来长期优势。考虑到每年生产和使用大量此类检测试剂,我们提出了一种用于抗体型生物标记传感器的高灵敏度碳电极的直接制造工艺。该工艺利用移印技术--一种添加剂、低温、低成本的方法,再加上等离子活化--证明了电极测量干扰素γ蛋白(一种结核病生物标记物)的能力。利用电化学阻抗光谱法,电极显示出很高的灵敏度,能够在一小时内测量未稀释血清中 10 至 1000 pg mL-1 的浓度。该传感器仅使用了一层抗体,其性能与本研究中测试的商用标准夹心酶联免疫吸附法相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信