Feasibility and effectiveness of transcutaneous auricular vagus nerve stimulation (taVNS) in awake mice

IF 4.8 1区医学 Q1 NEUROSCIENCES

CNS Neuroscience & Therapeutics Pub Date : 2024-09-11 DOI:10.1111/cns.70043

Yu-Mei Yu, Rui Yao, Zhou-Liang Liu, Yao Lu, Yang-Zi Zhu, Jun-Li Cao

{"title":"Feasibility and effectiveness of transcutaneous auricular vagus nerve stimulation (taVNS) in awake mice","authors":"Yu-Mei Yu, Rui Yao, Zhou-Liang Liu, Yao Lu, Yang-Zi Zhu, Jun-Li Cao","doi":"10.1111/cns.70043","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aims</h3>\n \n <p>Transcutaneous auricular vagus nerve stimulation (taVNS) is widely used to treat a variety of disorders because it is noninvasive, safe, and well tolerated by awake patients. However, long-term and repetitive taVNS is difficult to achieve in awake mice. Therefore, developing a new taVNS method that fully mimics the method used in clinical settings and is well-tolerated by awake mice is greatly important for generalizing research findings related to the effects of taVNS. The study aimed to develop a new taVNS device for use in awake mice and to test its reliability and effectiveness.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>We demonstrated the reliability of this taVNS device through retrograde neurotropic pseudorabies virus (PRV) tracing and evaluated its effectiveness through morphological analysis. After 3 weeks of taVNS application, the open field test (OFT) and elevated plus maze (EPM) were used to evaluate anxiety-like behaviors, and the Y-maze test and novel object recognition test (NORT) were used to evaluate recognition memory behaviors, respectively.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>We found that repetitive taVNS was well tolerated by awake mice, had no effect on anxiety-like behaviors, and significantly improved memory.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Our findings suggest that this new taVNS device for repetitive stimulation of awake mice is safe, tolerable, and effective.</p>\n </section>\n </div>","PeriodicalId":154,"journal":{"name":"CNS Neuroscience & Therapeutics","volume":"30 9","pages":""},"PeriodicalIF":4.8000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cns.70043","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"CNS Neuroscience & Therapeutics","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cns.70043","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Aims

Transcutaneous auricular vagus nerve stimulation (taVNS) is widely used to treat a variety of disorders because it is noninvasive, safe, and well tolerated by awake patients. However, long-term and repetitive taVNS is difficult to achieve in awake mice. Therefore, developing a new taVNS method that fully mimics the method used in clinical settings and is well-tolerated by awake mice is greatly important for generalizing research findings related to the effects of taVNS. The study aimed to develop a new taVNS device for use in awake mice and to test its reliability and effectiveness.

Methods

We demonstrated the reliability of this taVNS device through retrograde neurotropic pseudorabies virus (PRV) tracing and evaluated its effectiveness through morphological analysis. After 3 weeks of taVNS application, the open field test (OFT) and elevated plus maze (EPM) were used to evaluate anxiety-like behaviors, and the Y-maze test and novel object recognition test (NORT) were used to evaluate recognition memory behaviors, respectively.

Results

We found that repetitive taVNS was well tolerated by awake mice, had no effect on anxiety-like behaviors, and significantly improved memory.

Conclusion

Our findings suggest that this new taVNS device for repetitive stimulation of awake mice is safe, tolerable, and effective.

Abstract Image

查看原文本刊更多论文

清醒小鼠经皮耳廓迷走神经刺激（taVNS）的可行性和有效性

目的经皮耳廓迷走神经刺激（taVNS）具有无创、安全、清醒患者耐受性好等特点，被广泛用于治疗各种疾病。然而，在清醒的小鼠身上很难实现长期和重复的迷走神经刺激。因此，开发一种新的 taVNS 方法，使其完全模拟临床环境中使用的方法，并能被清醒的小鼠很好地耐受，这对推广与 taVNS 效果相关的研究成果非常重要。本研究旨在开发一种用于清醒小鼠的新型 taVNS 装置，并测试其可靠性和有效性。方法我们通过逆行神经伪狂犬病毒（PRV）追踪证明了这种 taVNS 装置的可靠性，并通过形态学分析评估了其有效性。应用taVNS 3周后，分别用开阔地试验（OFT）和高架迷宫（EPM）评估焦虑样行为，用Y-迷宫试验和新物体识别试验（NORT）评估识别记忆行为。结果我们发现，清醒小鼠对重复使用 taVNS 有良好的耐受性，对焦虑样行为没有影响，并能显著改善记忆。结论我们的研究结果表明，这种用于重复刺激清醒小鼠的新型 taVNS 装置是安全、可耐受和有效的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

CNS Neuroscience & Therapeutics 医学-神经科学

CiteScore

7.30

自引率

12.70%

发文量

240

审稿时长

2 months

期刊介绍： CNS Neuroscience & Therapeutics provides a medium for rapid publication of original clinical, experimental, and translational research papers, timely reviews and reports of novel findings of therapeutic relevance to the central nervous system, as well as papers related to clinical pharmacology, drug development and novel methodologies for drug evaluation. The journal focuses on neurological and psychiatric diseases such as stroke, Parkinson’s disease, Alzheimer’s disease, depression, schizophrenia, epilepsy, and drug abuse.