Lack of effects of polystyrene micro- and nanoplastics on activity and expression of human drug transporters

IF 5.4 3区 材料科学 Q2 CHEMISTRY, PHYSICAL
Valentin Tastet , Marc Le Vée , Alexis Verger , Nolwenn Brandhonneur , Arnaud Bruyère , Olivier Fardel
{"title":"Lack of effects of polystyrene micro- and nanoplastics on activity and expression of human drug transporters","authors":"Valentin Tastet ,&nbsp;Marc Le Vée ,&nbsp;Alexis Verger ,&nbsp;Nolwenn Brandhonneur ,&nbsp;Arnaud Bruyère ,&nbsp;Olivier Fardel","doi":"10.1016/j.etap.2024.104563","DOIUrl":null,"url":null,"abstract":"<div><p>Micro- and nanoplastics (MPs/NPs) constitute emerging and widely-distributed environmental contaminants to which humans are highly exposed. They possibly represent a threat for human health. In order to identify cellular/molecular targets for these plastic particles, we have analysed the effects of exposure to manufactured polystyrene (PS) MPs and NPs on <em>in vitro</em> activity and expression of human membrane drug transporters, known to interact with chemical pollutants. PS MPs and NPs, used at various concentrations (1, 10 or 100 µg/mL), failed to inhibit efflux activities of the ATP-binding cassette (ABC) transporters P-glycoprotein, MRPs and BCRP in ABC transporter-expressing cells. Furthermore, PS particles did not impair the transport of P-glycoprotein or BCRP substrates across intestinal Caco-2 cell monolayers. Uptake activities of solute carriers (SLCs) such as OCT1 and OCT2 (handling organic cations) or OATP1B1, OATP1B3, OATP2B1, OAT1 and OAT3 (handling organic anions) were additionally not altered by PS MPs/NPs in HEK-293 cells overexpressing these SLCs. mRNA expression of ABC transporters and of the SLCs OCT1 and OATP2B1 in Caco-2 cells and human hepatic HepaRG cells were finally not impaired by a 48-h exposure to MPs/NPs. Altogether, these data indicate that human drug transporters are unlikely to be direct and univocal targets for synthetic PS MPs/NPs.</p></div>","PeriodicalId":4,"journal":{"name":"ACS Applied Energy Materials","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1382668924002035/pdfft?md5=4dfd6e7edfb017440af51db88ecdc5eb&pid=1-s2.0-S1382668924002035-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Energy Materials","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1382668924002035","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Micro- and nanoplastics (MPs/NPs) constitute emerging and widely-distributed environmental contaminants to which humans are highly exposed. They possibly represent a threat for human health. In order to identify cellular/molecular targets for these plastic particles, we have analysed the effects of exposure to manufactured polystyrene (PS) MPs and NPs on in vitro activity and expression of human membrane drug transporters, known to interact with chemical pollutants. PS MPs and NPs, used at various concentrations (1, 10 or 100 µg/mL), failed to inhibit efflux activities of the ATP-binding cassette (ABC) transporters P-glycoprotein, MRPs and BCRP in ABC transporter-expressing cells. Furthermore, PS particles did not impair the transport of P-glycoprotein or BCRP substrates across intestinal Caco-2 cell monolayers. Uptake activities of solute carriers (SLCs) such as OCT1 and OCT2 (handling organic cations) or OATP1B1, OATP1B3, OATP2B1, OAT1 and OAT3 (handling organic anions) were additionally not altered by PS MPs/NPs in HEK-293 cells overexpressing these SLCs. mRNA expression of ABC transporters and of the SLCs OCT1 and OATP2B1 in Caco-2 cells and human hepatic HepaRG cells were finally not impaired by a 48-h exposure to MPs/NPs. Altogether, these data indicate that human drug transporters are unlikely to be direct and univocal targets for synthetic PS MPs/NPs.

聚苯乙烯微塑料和纳米塑料对人体药物转运体的活性和表达没有影响
微塑料和纳米塑料(MPs/NPs)是新出现的、分布广泛的环境污染物,人类与它们的接触程度很高。它们可能对人类健康构成威胁。为了确定这些塑料微粒的细胞/分子靶标,我们分析了暴露于人造聚苯乙烯(PS)MPs 和 NPs 对已知会与化学污染物相互作用的人类膜药物转运体的体外活性和表达的影响。不同浓度(1、10 或 100 µg/mL)的聚苯乙烯 MPs 和 NPs 未能抑制 ATP 结合盒(ABC)转运体 P-糖蛋白、MRPs 和 BCRP 在 ABC 转运体表达细胞中的外流活性。此外,PS 颗粒不会影响 P 糖蛋白或 BCRP 底物在肠道 Caco-2 细胞单层中的转运。此外,在过表达 OCT1 和 OCT2(处理有机阳离子)或 OATP1B1、OATP1B3、OATP2B1、OAT1 和 OAT3(处理有机阴离子)等溶质载体(SLC)的 HEK-293 细胞中,PS MPs/NPs 也不会改变这些 SLC 的吸收活性。Caco-2细胞和人类肝脏HepaRG细胞中ABC转运体以及SLC OCT1和OATP2B1的mRNA表达最终也没有因为暴露于MPs/NPs 48小时而受到影响。总之,这些数据表明,人类药物转运体不太可能成为合成 PS MPs/NPs 的直接和唯一靶标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
ACS Applied Energy Materials
ACS Applied Energy Materials Materials Science-Materials Chemistry
CiteScore
10.30
自引率
6.20%
发文量
1368
期刊介绍: ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信