Universal sulfatase-based chemiluminescence biosensing platform: Validation via AFP detection in clinical blood samples

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2024-09-10 DOI:10.1016/j.bios.2024.116771

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引用次数: 0

Abstract

Enzyme-catalyzed chemiluminescence has been widely used in the field of biomedicine, especially in the test kit for various biomarkers. However, the currently reported enzyme-catalyzed chemiluminescence systems suffered from the addition of oxidizing substances, short emission wavelength, and susceptibility to interference by autofluorescence. In this paper, a universal sulfatase-based chemiluminescence system with NIR was developed, in which the designed substrate QM-CF could be transformed into 1,2-dioxetane derivate in the presence of sulfatase and oxygen. This system exhibited long emission wavelengths and CL half-time, a high signal-noise ratio, and without other additives. Importantly, the sulfatase-based chemiluminescence enzyme-linked immunoassay platform was successfully constructed and could be generally applied to detect biomarkers. As a proof of concept, the sulfatase-labeled AFP antibody and substate QM-CF were conveniently suitable for commercial AFP test kits, leading to satisfactory detection results of AFP in clinical blood samples.

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基于硫酸酯酶的通用化学发光生物传感平台：通过检测临床血液样本中的甲胎蛋白进行验证

酶催化化学发光已广泛应用于生物医学领域，尤其是各种生物标记物的检测试剂盒中。然而，目前报道的酶催化化学发光系统存在添加氧化性物质、发射波长短、易受自发荧光干扰等问题。本文开发了一种基于硫酸亚铁酶的通用近红外化学发光系统，其中设计的底物 QM-CF 可在硫酸亚铁酶和氧气的存在下转化为 1,2-二氧杂环丁烷衍生物。该系统具有发射波长长、CL 半衰期长、信噪比高的特点，而且无需其他添加剂。重要的是，基于硫酸酯酶的化学发光酶联免疫分析平台已成功构建，并可普遍应用于检测生物标记物。作为概念验证，硫酸酯酶标记的甲胎蛋白抗体和亚基QM-CF可方便地用于甲胎蛋白检测试剂盒，从而在临床血液样本中获得令人满意的甲胎蛋白检测结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.