Segmental patterning of microbiota and immune cells in the murine intestinal tract.

IF 12.2 1区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Microbes Pub Date : 2024-09-10 DOI:10.1080/19490976.2024.2398126

Harithaa Anandakumar,Ariana Rauch,Moritz I Wimmer,Alex Yarritu,Gudrun Koch,Victoria McParland,Hendrik Bartolomaeus,Nicola Wilck

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引用次数: 0

Abstract

The intestine exhibits distinct characteristics along its length, with a substantial immune cell reservoir and diverse microbiota crucial for maintaining health. This study investigates how anatomical location and regional microbiota influence intestinal immune cell abundance. Using conventionally colonized and germ-free mice, segment-specific immune cell composition and microbial communities were assessed. Metagenomic sequencing analyzed microbiome variations, while flow cytometry and immunofluorescence examined immune cell composition. Microbiome composition varied significantly along the intestine, with diversity and abundance increasing from upper to lower segments. Immune cells showed distinct segment-specific patterning influenced by microbial colonization and localization. T cell subsets displayed varied dependence on microbiome presence and anatomical location. This study highlights locoregional differences in intestinal immune cell and microbiome composition, identifying immune subsets susceptible to microbiota presence. The findings provide context for understanding immune cell alterations in disease models.

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小鼠肠道微生物群和免疫细胞的节段模式。

肠道沿其长度方向呈现出不同的特征，其中大量的免疫细胞库和多样化的微生物群对维持健康至关重要。本研究探讨了解剖位置和区域微生物群如何影响肠道免疫细胞的丰度。研究人员使用常规定植小鼠和无菌小鼠，对特定区段的免疫细胞组成和微生物群落进行了评估。元基因组测序分析了微生物群的变化，流式细胞术和免疫荧光检查了免疫细胞的组成。微生物组的组成在肠道内有明显的差异，从上段到下段，多样性和丰度都在增加。免疫细胞受微生物定植和定位的影响，表现出明显的区段特异性模式。T 细胞亚群显示出对微生物群存在和解剖位置的不同依赖性。这项研究强调了肠道免疫细胞和微生物群组成的局部区域差异，确定了易受微生物群存在影响的免疫亚群。这些发现为了解疾病模型中免疫细胞的改变提供了背景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gut Microbes Medicine-Microbiology (medical)

CiteScore

18.20

自引率

3.30%

发文量

196

审稿时长

10 weeks

期刊介绍： The intestinal microbiota plays a crucial role in human physiology, influencing various aspects of health and disease such as nutrition, obesity, brain function, allergic responses, immunity, inflammatory bowel disease, irritable bowel syndrome, cancer development, cardiac disease, liver disease, and more. Gut Microbes serves as a platform for showcasing and discussing state-of-the-art research related to the microorganisms present in the intestine. The journal emphasizes mechanistic and cause-and-effect studies. Additionally, it has a counterpart, Gut Microbes Reports, which places a greater focus on emerging topics and comparative and incremental studies.