In vivo spatiotemporal characterizing diverse body transportation of optical labeled high immunity aluminium adjuvants with photoacoustic tomography

IF 7.1 1区医学 Q1 ENGINEERING, BIOMEDICAL

Photoacoustics Pub Date : 2024-09-07 DOI:10.1016/j.pacs.2024.100643

Fan Meng , Chaohao Liang , Barkat Ali , Changwu Wan , Fengbing He , Jiarui Chen , Yiqing Zhang , Zhijia Luo , Lingling Su , Xiaoya Zhao , Bin Yang , Jian Zhang

{"title":"In vivo spatiotemporal characterizing diverse body transportation of optical labeled high immunity aluminium adjuvants with photoacoustic tomography","authors":"Fan Meng , Chaohao Liang , Barkat Ali , Changwu Wan , Fengbing He , Jiarui Chen , Yiqing Zhang , Zhijia Luo , Lingling Su , Xiaoya Zhao , Bin Yang , Jian Zhang","doi":"10.1016/j.pacs.2024.100643","DOIUrl":null,"url":null,"abstract":"<div><p>Vaccine development requires high-resolution, in situ, and visual adjuvant technology. To address this need, this work proposed a novel adjuvant labeling that involved indocyanine green (ICG) and bovine serum albumin (BSA) with self-assembled aluminium adjuvant (Alum), which was called BSA@ICG@Alum. This compound exhibited excellent photoacoustic properties and has been confirmed its safety, biocompatibility, high antigen binding efficiency, and superior induction of immune response. Photoacoustic tomography (PAT) tracked the distribution of Alum in lymph nodes (LNs) and lymphatic vessels in real time after diverse injection modalities. The non-invasive imaging approach revealed that BSA@ICG@Alum was transported to the draining LNs 60 min after intramuscular injection and to distal LNs within 30 min after lymph node injection. In conclusion, PAT enabled real-time three-dimensional and quantitative visualization, thus offering a powerful tool for advancing vaccine design by providing critical insights into adjuvant transport and immune system activation.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"39 ","pages":"Article 100643"},"PeriodicalIF":7.1000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000600/pdfft?md5=3d8b3dbac9426821f6cdb75e43d7fccf&pid=1-s2.0-S2213597924000600-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photoacoustics","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213597924000600","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Vaccine development requires high-resolution, in situ, and visual adjuvant technology. To address this need, this work proposed a novel adjuvant labeling that involved indocyanine green (ICG) and bovine serum albumin (BSA) with self-assembled aluminium adjuvant (Alum), which was called BSA@ICG@Alum. This compound exhibited excellent photoacoustic properties and has been confirmed its safety, biocompatibility, high antigen binding efficiency, and superior induction of immune response. Photoacoustic tomography (PAT) tracked the distribution of Alum in lymph nodes (LNs) and lymphatic vessels in real time after diverse injection modalities. The non-invasive imaging approach revealed that BSA@ICG@Alum was transported to the draining LNs 60 min after intramuscular injection and to distal LNs within 30 min after lymph node injection. In conclusion, PAT enabled real-time three-dimensional and quantitative visualization, thus offering a powerful tool for advancing vaccine design by providing critical insights into adjuvant transport and immune system activation.

查看原文本刊更多论文

利用光声断层扫描技术对光学标记的高免疫性铝佐剂的体内时空迁移进行表征

疫苗开发需要高分辨率、原位和可视佐剂技术。为满足这一需求，本研究提出了一种新型佐剂标记方法，即吲哚菁绿（ICG）和牛血清白蛋白（BSA）与自组装铝佐剂（Alum）的结合，称为 BSA@ICG@Alum。该化合物具有优异的光声特性，其安全性、生物相容性、高抗原结合效率和诱导免疫反应的能力已得到证实。光声断层扫描（PAT）可实时追踪明矾在不同注射方式后在淋巴结和淋巴管中的分布情况。这种非侵入性成像方法显示，BSA@ICG@Alum 在肌肉注射 60 分钟后被输送到引流淋巴结，并在淋巴结注射后 30 分钟内被输送到远端淋巴结。总之，PAT 实现了实时三维和定量可视化，从而为推进疫苗设计提供了强大的工具，为佐剂运输和免疫系统激活提供了重要的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Photoacoustics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

11.40

自引率

16.50%

发文量

审稿时长

53 days

期刊介绍： The open access Photoacoustics journal (PACS) aims to publish original research and review contributions in the field of photoacoustics-optoacoustics-thermoacoustics. This field utilizes acoustical and ultrasonic phenomena excited by electromagnetic radiation for the detection, visualization, and characterization of various materials and biological tissues, including living organisms. Recent advancements in laser technologies, ultrasound detection approaches, inverse theory, and fast reconstruction algorithms have greatly supported the rapid progress in this field. The unique contrast provided by molecular absorption in photoacoustic-optoacoustic-thermoacoustic methods has allowed for addressing unmet biological and medical needs such as pre-clinical research, clinical imaging of vasculature, tissue and disease physiology, drug efficacy, surgery guidance, and therapy monitoring. Applications of this field encompass a wide range of medical imaging and sensing applications, including cancer, vascular diseases, brain neurophysiology, ophthalmology, and diabetes. Moreover, photoacoustics-optoacoustics-thermoacoustics is a multidisciplinary field, with contributions from chemistry and nanotechnology, where novel materials such as biodegradable nanoparticles, organic dyes, targeted agents, theranostic probes, and genetically expressed markers are being actively developed. These advanced materials have significantly improved the signal-to-noise ratio and tissue contrast in photoacoustic methods.