Unveiling the genetic landscape of infectious laryngotracheitis virus in Switzerland: Evidence for vaccine-like and wild-type strains

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2024-09-05 DOI:10.1016/j.virol.2024.110217

Sonja Hermann , Marc J.A. Stevens , Brigitte Sigrist , Ivana Bilic , Sarah Albini , Nina Wolfrum

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Abstract

Infectious laryngotracheitis (ILT) is a respiratory disease affecting chickens worldwide. Unlike many countries, Switzerland does not vaccinate against ILT. This study analysed ILT samples from 21 natural outbreaks in Switzerland using restriction fragment length polymorphism (RFLP) and multiple gene sequencing. Chicken embryo origin (CEO) and tissue culture origin (TCO) vaccine strains were included as references. Both vaccine strains were distinguishable, and 14 out of 21 samples resembled the CEO vaccine. Additionally, four distinct non-vaccine-like groups were identified. Sequencing of three genes from selected Swiss samples and those from neighbouring countries revealed four phylogenetic clades. Notably, four Swiss field strains formed two unique clades, not closely related to vaccine strains or ILTV from neighbouring countries. Overall, RFLP results were supported by sequencing data. This study demonstrates the presence of both vaccine-like and wild-type ILT viruses in Switzerland, where vaccination is de facto prohibited.

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揭示瑞士传染性喉气管炎病毒的基因状况：疫苗样株和野生型株的证据

传染性喉气管炎 (ILT) 是一种影响全球鸡只的呼吸道疾病。与许多国家不同，瑞士不接种 ILT 疫苗。本研究利用限制性片段长度多态性（RFLP）和多基因测序分析了瑞士 21 次自然爆发的 ILT 样本。鸡胚来源（CEO）和组织培养来源（TCO）疫苗株被列为参照物。两种疫苗菌株均可区分，21 个样本中有 14 个与 CEO 疫苗相似。此外，还发现了 4 个不同的非疫苗相似群。对所选瑞士样本和邻国样本的三个基因进行测序，发现了四个系统发育支系。值得注意的是，四个瑞士野外菌株形成了两个独特的支系，与疫苗菌株或邻国的 ILTV 没有密切关系。总体而言，RFLP 结果得到了测序数据的支持。这项研究表明，在事实上禁止接种疫苗的瑞士，既存在疫苗样病毒，也存在野生型ILT病毒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： The journal features articles on virus replication, virus-host biology, viral pathogenesis, immunity to viruses, virus structure, and virus evolution and ecology. We aim to publish papers that provide advances to the understanding of virus biology.