Innovative approaches in invertase immobilization: Utilizing green synthesized zinc oxide nanoparticles to improve biochemical properties

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-09-07 DOI:10.1016/j.ab.2024.115661

Somayeh Sadi , Marzieh Ghollasi , Khadijeh Eskandari , Elahe Darvishi

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引用次数: 0

Abstract

Invertase enzyme can effectively improve the taste, color, and durability of these products. Various methods have been proposed to increase the stability and efficiency of enzymes. One of the most important is enzyme immobilization, which can be implemented on different materials. The purpose of this study was to immobilize the invertase enzyme on the surface of green synthesized zinc oxide nanoparticles and to investigate its biochemical properties. The enzyme immobilization was confirmed by SEM and Raman spectroscopy. Then, the biochemical characteristics, such as optimal pH and temperature, thermal stability, and storage stability of free and immobilized enzymes, were determined. The results of SEM showed that the diameter of synthesized nanoparticles was about 60 ± 5 nm. FTIR of immobilized invertase confirmed the immobilization process. The immobilization efficiency was determined to be 72 %. Immobilized enzyme showed higher thermal stability at 40 and 50 °C. Immobilized enzyme could be used 8 times in optimum condition. Also, an Examination of the kinetic parameters of the immobilized enzyme compared with those of the free enzyme showed a decrease in the maximum velocity of the enzyme. It seems that the immobilized invertase has improved characteristics for application in different industries.

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转化酶固定化的创新方法：利用绿色合成的氧化锌纳米粒子改善生化特性

转化酶能有效改善这些产品的口感、色泽和耐久性。人们提出了各种方法来提高酶的稳定性和效率。其中最重要的是酶的固定化，它可以在不同的材料上实现。本研究的目的是将转化酶固定在绿色合成的氧化锌纳米粒子表面，并研究其生化特性。通过扫描电镜和拉曼光谱确认了酶的固定。然后，测定了游离酶和固定化酶的最佳 pH 值和温度、热稳定性和储存稳定性等生化特性。扫描电镜结果显示，合成的纳米颗粒直径约为 60 ± 5 nm。固定化转化酶的傅立叶变换红外光谱证实了固定化过程。经测定，固定化效率为 72%。固定化酶在 40 和 50 °C下表现出较高的热稳定性。在最佳条件下，固定化酶可使用 8 次。此外，与游离酶相比，对固定化酶的动力学参数进行的检查显示，酶的最大速度有所下降。由此看来，固定化转化酶具有更好的特性，可以应用于不同的行业。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.