Waste-derived Bhetki Fish (Lates calcarifer) dermal collagen and Mn, Zn doped bioactive glass composite electrospun mats as a synergistic approach to enhance wound healing

IF 7.1 3区材料科学 Q1 GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY

Materials Today Sustainability Pub Date : 2024-09-05 DOI:10.1016/j.mtsust.2024.100979

Chaudhuri Mohammad Tarif , Pratik Das , Tuhin Sarkar , Pradyot Datta , Prasenjit Mukherjee , Samiran Mondal , Subhasis Roy , Piyali Basak , Biswanath Kundu , Samit Kumar Nandi

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Abstract

Critical wounds require large-scale, low-cost treatments to restore damaged tissue and function. This work aims to investigate the potential of bioactive glasses with Bhetki (Lates calcarifer) skin-derived collagen in wound healing. SDS-PAGE analysis, UV-VIS, and FTIR spectra identify the isolated Bhetki fish collagen as type 1 collagen. The collagen is subsequently mixed into bioactive glass compositions (BAG, Mn-BAG, Zn-BAG, and Mn–Zn BAG) to develop electrospun mats. FTIR and XRD characterization confirms the successful combination of collagen with bioactive glass. SEM analysis revealed homogeneous, electrospun microfibrous mats with sub-micron to micro-sized fibers, highly porous interconnected networks, and EDX-confirmed elemental composition (C, N, O, Si, Mn, Zn), indicating successful BAG matrix doping. The antibacterial activity assessment revealed the efficacy of mats containing manganese (Mn), zinc (Zn), or a combination against Escherichia coli and Staphylococcus aureus. Cytocompatibility studies with L929 cells showed good cell proliferation. In a rabbit model, the mats, particularly the BFCol/MnZnBAG, demonstrated accelerated wound healing, with significant wound closure from 46.97% on day 3–4.77% on day 14, well-organized collagenous structures, and enhanced neovascularization as shown by CD31 positive staining. The findings suggest that these composite mats, especially the ion-doped variants, hold great promise for effective wound healing.

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从废弃物中提取的贝特基鱼（Lates calcarifer）真皮胶原蛋白和掺杂锰、锌的生物活性玻璃复合电纺垫可协同促进伤口愈合

严重的伤口需要大规模、低成本的治疗来恢复受损组织和功能。这项工作旨在研究生物活性玻璃与 Bhetki（鳐鱼）皮源胶原蛋白在伤口愈合中的潜力。通过 SDS-PAGE 分析、紫外可见光谱和傅立叶变换红外光谱，确定分离出的 Bhetki 鱼胶原蛋白为 1 型胶原蛋白。随后将胶原蛋白与生物活性玻璃成分（BAG、Mn-BAG、Zn-BAG 和 Mn-Zn BAG）混合，制成电纺垫。傅立叶变换红外光谱和 XRD 表征证实了胶原蛋白与生物活性玻璃的成功结合。扫描电子显微镜分析表明，电纺微纤维毡具有亚微米到微米大小的纤维、高度多孔的互连网络以及经 EDX 证实的元素组成（C、N、O、Si、Mn、Zn），表明 BAG 基质掺杂成功。抗菌活性评估显示，含有锰（Mn）、锌（Zn）或其组合的毡对大肠杆菌和金黄色葡萄球菌具有抗菌效果。用 L929 细胞进行的细胞相容性研究表明，细胞增殖良好。在兔子模型中，这些垫子，尤其是 BFCol/MnZnBAG 显示伤口愈合加快，第 3-4 天伤口闭合率从 46.97% 显著提高到 14.77%，胶原结构组织良好，CD31 阳性染色显示新生血管增强。研究结果表明，这些复合垫，尤其是离子掺杂的变体，有望实现有效的伤口愈合。

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来源期刊

Materials Today Sustainability Multiple-

CiteScore

5.80

自引率

6.40%

发文量

174

审稿时长

32 days

期刊介绍： Materials Today Sustainability is a multi-disciplinary journal covering all aspects of sustainability through materials science. With a rapidly increasing population with growing demands, materials science has emerged as a critical discipline toward protecting of the environment and ensuring the long term survival of future generations.