Single nucleus/cell RNA-seq of the chicken hypothalamic-pituitary-ovarian axis offers new insights into the molecular regulatory mechanisms of ovarian development.

IF 4 1区 生物学 Q1 ZOOLOGY
Dong Leng, Bo Zeng, Tao Wang, Bin-Long Chen, Di-Yan Li, Zhuan-Jian Li
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Abstract

The hypothalamic-pituitary-ovarian (HPO) axis represents a central neuroendocrine network essential for reproductive function. Despite its critical role, the intrinsic heterogeneity within the HPO axis across vertebrates and the complex intercellular interactions remain poorly defined. This study provides the first comprehensive, unbiased, cell type-specific molecular profiling of all three components of the HPO axis in adult Lohmann layers and Liangshan Yanying chickens. Within the hypothalamus, pituitary, and ovary, seven, 12, and 13 distinct cell types were identified, respectively. Results indicated that the pituitary adenylate cyclase activating polypeptide (PACAP), follicle-stimulating hormone (FSH), and prolactin (PRL) signaling pathways may modulate the synthesis and secretion of gonadotropin-releasing hormone (GnRH), FSH, and luteinizing hormone (LH) within the hypothalamus and pituitary. In the ovary, interactions between granulosa cells and oocytes involved the KIT, CD99, LIFR, FN1, and ANGPTL signaling pathways, which collectively regulate follicular maturation. The SEMA4 signaling pathway emerged as a critical mediator across all three tissues of the HPO axis. Additionally, gene expression analysis revealed that relaxin 3 (RLN3), gastrin-releasing peptide (GRP), and cocaine- and amphetamine regulated transcripts (CART, also known as CARTPT) may function as novel endocrine hormones, influencing the HPO axis through autocrine, paracrine, and endocrine pathways. Comparative analyses between Lohmann layers and Liangshan Yanying chickens demonstrated higher expression levels of GRP, RLN3, CARTPT, LHCGR, FSHR, and GRPR in the ovaries of Lohmann layers, potentially contributing to their superior reproductive performance. In conclusion, this study provides a detailed molecular characterization of the HPO axis, offering novel insights into the regulatory mechanisms underlying reproductive biology.

鸡下丘脑-垂体-卵巢轴的单核/细胞 RNA 序列分析为卵巢发育的分子调控机制提供了新的视角。
下丘脑-垂体-卵巢(HPO)轴是对生殖功能至关重要的中枢神经内分泌网络。尽管下丘脑-垂体-卵巢轴发挥着关键作用,但其在脊椎动物中的内在异质性和复杂的细胞间相互作用仍鲜为人知。本研究首次对成年罗曼蛋鸡和凉山盐化鸡 HPO 轴的所有三个组成部分进行了全面、无偏见、细胞类型特异性的分子图谱分析。在下丘脑、垂体和卵巢中,分别发现了 7、12 和 13 种不同的细胞类型。结果表明,垂体腺苷酸环化酶激活多肽(PACAP)、卵泡刺激素(FSH)和催乳素(PRL)信号通路可调节下丘脑和垂体内促性腺激素释放激素(GnRH)、FSH和黄体生成素(LH)的合成和分泌。在卵巢中,颗粒细胞和卵母细胞之间的相互作用涉及 KIT、CD99、LIFR、FN1 和 ANGPTL 信号通路,它们共同调节卵泡的成熟。SEMA4 信号通路是 HPO 轴上所有三个组织的关键介质。此外,基因表达分析表明,弛缓素 3(RLN3)、胃泌素释放肽(GRP)以及可卡因和苯丙胺调控转录物(CART,又称 CARTPT)可作为新型内分泌激素发挥作用,通过自分泌、旁分泌和内分泌途径影响 HPO 轴。罗曼蛋鸡和凉山盐肤鸡的比较分析表明,罗曼蛋鸡卵巢中的GRP、RLN3、CARTPT、LHCGR、FSHR和GRPR的表达水平更高,这可能是其繁殖性能更优越的原因。总之,本研究提供了 HPO 轴的详细分子特征,为生殖生物学的基础调控机制提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Zoological Research
Zoological Research Medicine-General Medicine
CiteScore
7.60
自引率
10.20%
发文量
1937
审稿时长
8 weeks
期刊介绍: Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.
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