Peptide-based immunoprotection against Rhipicephalus microplus tick

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2024-08-25 DOI:10.1016/j.vetpar.2024.110294

Renato Andreotti , Marcos Valério Garcia , Jacqueline Cavalcante Barros , Leandra Marla Oshiro , Namor Pinheiro Zimmermann , Leandro de Oliveira Souza Higa , Pâmella Oliveira Duarte , Rodrigo Casquero Cunha

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引用次数: 0

Abstract

The main agents for tick control are chemical acaricides. However, when used without technical guidance, they can lead to environmental damage and the development of resistant tick strains. In this context, vaccines are alternative o be used in integrated tick management format by combining with other effective tools. We isolated RNA from ticks Rhipicephalus microplus, prepared the library, and performed next-generation sequencing; a pipeline analysis was applied to identify the hypothetical proteins having immunogenic potential and their predicted immunogenic peptides. Twelve peptides, ranging from 12 to 38 amino acid residues, containing the selected epitopes from different targets were selected and synthesized in two forms: the pure peptide; and the peptide conjugated to keyhole limpet hemocyanin (KLH) carrier. These peptides were divided into two groups of six peptides each. The antigen formulations (groups 1 and 2) were prepared with conjugated peptides containing 200 µg of each peptide per dose emulsified with Montanide ISA 61VG (SEPPIC); the control treatment had the adjuvant formulation without peptides (group 3). To evaluate the protective efficacy, 15 weaned male calves (Angus breed) aged around 6 months to one year and weighing approximately 200–250 kg were divided into three groups of five animals each; they were immunized thrice, at an interval of 28 days. After immunization, all the calves infested with 15,000 larvae of Rhipicephalus microplus. Peptide epitopes were recognized by antibodies against host-specific IgGs using indirect ELISA. The mean of the antibody level was determined for each group and compared using analysis of variance with two factors (ANOVA). F-test was used to determine the significance of differences observed between the groups. The percentage efficacy was calculated based on the number of ticks, the weight of teleoginas, and the weight and hatchability of the eggs, compared to that in the control group. The evaluation of immunoprotection indicated efficacies of 69 and 51 %, respectively in Group 1 and 2.

查看原文本刊更多论文

基于肽的免疫保护剂可防 Rhipicephalus microplus 蜱虫。

控制蜱虫的主要药剂是化学杀螨剂。但是，如果在没有技术指导的情况下使用，可能会导致环境破坏和抗药性蜱株的发展。在这种情况下，疫苗是一种替代品，可与其他有效工具相结合，用于蜱虫综合治理。我们从蜱Rhipicephalus microplus中分离出RNA，制备文库，并进行了新一代测序；应用流水线分析确定了具有免疫原性潜力的假定蛋白及其预测的免疫原性肽。研究人员从不同的靶标中筛选出含有所选表位的 12 至 38 个氨基酸残基的 12 种肽段，并合成了两种形式的肽段：纯肽段和与匙孔帽形血蓝蛋白（KLH）载体共轭的肽段。这些肽分为两组，每组六条肽。抗原制剂（第 1 组和第 2 组）是用孟他尼 ISA 61VG （SEPPIC）乳化的共轭肽制剂，每剂含 200 µg 肽；对照组是不含肽的佐剂制剂（第 3 组）。为了评估保护效力，15 头断奶的雄性小牛（安格斯品种）被分成三组，每组 5 头，年龄在 6 个月到 1 岁之间，体重约为 200-250 千克；每组免疫 3 次，每次间隔 28 天。免疫后，所有犊牛都感染了 15,000 头小尾寒羊幼虫。用间接酶联免疫吸附法检测宿主特异性 IgG 对肽表位的识别。确定各组抗体水平的平均值，并使用双因素方差分析（ANOVA）进行比较。F 检验用于确定观察到的组间差异的显著性。与对照组相比，根据蜱的数量、蜱卵的重量、蜱卵的重量和孵化率计算疗效百分比。免疫保护评估表明，第 1 组和第 2 组的有效率分别为 69% 和 51%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.