Modifications in the Composition of the Gut Microbiota in Rats Induced by Chronic Sleep Deprivation: Potential Relation to Mental Disorders.

IF 3 2区医学 Q2 CLINICAL NEUROLOGY

Nature and Science of Sleep Pub Date : 2024-09-04 eCollection Date: 2024-01-01 DOI:10.2147/NSS.S476691

Li-Ming Zheng, Yan Li

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引用次数: 0

Abstract

Introduction: Sleep deprivation(SD) has numerous negative effects on mental health. A growing body of research has confirmed the implication of gut microbiota in mental disorders. However, the specific modifications in mammalian gut microbiota following SD exhibit variations across different studies.

Methods: Male specific-pathogen-free Wistar rats were given a modified multiple-platform exposure for 7 days of SD. Fecal samples were obtained from the control and SD groups both at baseline and after 7 days of SD. We utilized 16S rDNA gene sequencing to investigate the gut microbial composition and functional pathways in rats.

Results: Analysis of the microbiota composition revealed a significant change in gut microbial composition after chronic SD, especially at the phylum level. The relative abundances of p_Firmicutes, g_Romboutsia, and g_Enterococcus increased, whereas those of p_Bacteroidetes, p_Verrucomicrobia, p_Fusobacteria, g_Akkermansia, and g_Cetobacterium decreased in animals after chronic SD compared with controls or animals before SD. The ratio of Firmicutes to Bacteroidetes exhibited an increase following SD. The relative abundance of gut microbiota related to the functional pathways of GABAergic and glutamatergic synapses was observed to be diminished in rats following SD compared to pre-SD.

Conclusion: Collectively, these findings suggest that chronic SD causes significant alterations in both the structural composition and functional pathways of the gut microbiome. Further researches are necessary to investigate the chronological and causal connections among SD, the gut microbiota and mental disorders.

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长期睡眠不足诱发大鼠肠道微生物群组成的改变：与精神疾病的潜在关系。

简介睡眠不足（SD）对心理健康有许多负面影响。越来越多的研究证实，肠道微生物群与精神疾病有关。然而，在不同的研究中，睡眠剥夺后哺乳动物肠道微生物群的具体变化情况各不相同：方法：对雄性无特定病原体的 Wistar 大鼠进行为期 7 天的改良多平台 SD 暴露。从对照组和 SD 组获取基线和 SD 7 天后的粪便样本。我们利用 16S rDNA 基因测序来研究大鼠的肠道微生物组成和功能途径：结果：微生物群组成分析表明，慢性 SD 后肠道微生物组成发生了显著变化，尤其是在门一级。与对照组或SD前的动物相比，慢性SD后的动物p_Firmicutes、g_Romboutsia和g_Enterococcus的相对丰度增加，而p_Bacteroidetes、p_Verrucomicrobia、p_Fusobacteria、g_Akermansia和g_Cetobacterium的相对丰度下降。固缩后，固缩菌与类杆菌的比例有所增加。观察到与 GABA 能和谷氨酸能突触的功能途径相关的肠道微生物群的相对丰度在 SD 后比 SD 前有所降低：总之，这些研究结果表明，慢性 SD 会导致肠道微生物群的结构组成和功能途径发生显著变化。有必要进一步研究 SD、肠道微生物群和精神疾病之间的时间和因果关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature and Science of Sleep Neuroscience-Behavioral Neuroscience

CiteScore

5.70

自引率

5.90%

发文量

245

审稿时长

16 weeks

期刊介绍： Nature and Science of Sleep is an international, peer-reviewed, open access journal covering all aspects of sleep science and sleep medicine, including the neurophysiology and functions of sleep, the genetics of sleep, sleep and society, biological rhythms, dreaming, sleep disorders and therapy, and strategies to optimize healthy sleep. Specific topics covered in the journal include: The functions of sleep in humans and other animals Physiological and neurophysiological changes with sleep The genetics of sleep and sleep differences The neurotransmitters, receptors and pathways involved in controlling both sleep and wakefulness Behavioral and pharmacological interventions aimed at improving sleep, and improving wakefulness Sleep changes with development and with age Sleep and reproduction (e.g., changes across the menstrual cycle, with pregnancy and menopause) The science and nature of dreams Sleep disorders Impact of sleep and sleep disorders on health, daytime function and quality of life Sleep problems secondary to clinical disorders Interaction of society with sleep (e.g., consequences of shift work, occupational health, public health) The microbiome and sleep Chronotherapy Impact of circadian rhythms on sleep, physiology, cognition and health Mechanisms controlling circadian rhythms, centrally and peripherally Impact of circadian rhythm disruptions (including night shift work, jet lag and social jet lag) on sleep, physiology, cognition and health Behavioral and pharmacological interventions aimed at reducing adverse effects of circadian-related sleep disruption Assessment of technologies and biomarkers for measuring sleep and/or circadian rhythms Epigenetic markers of sleep or circadian disruption.