The effect of Tyloxapol on the metabolome of Mycobacterium tuberculosis

IF 1.7 4区 生物学 Q4 BIOCHEMICAL RESEARCH METHODS
Monique Opperman , Ray-Dean Pietersen , Du Toit Loots , Mari van Reenen , Derylize Beukes , Bienyameen Baker , Ilse du Preez
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引用次数: 0

Abstract

The use of detergents when culturing Mycobacterium tuberculosis (M. tuberculosis) are essential to prevent clumping. However, these detergents may influence research outcomes by impacting bacterial morphology and metabolism. This study aimed to assess the metabolome of a M. tuberculosis H37Rv strain cultured with Tyloxapol (H37RvTyloxapol), compared to a control group of H37Rv strain cultured without detergent (H37RvControl) to evaluate Tyloxapol's suitability for metabolomic studies. Distinct metabolic alterations were observed in H37RvTyloxapol compared to H37RvControl, primarily associated with fatty acid, sugar and pentose phosphate metabolic pathways. These changes are associated with the surface stress exerted by Tyloxapol on the bacteria, prompting an adaptation of M. tuberculosis metabolism to that usually observed in stress environments. Nevertheless, the effect of Tyloxapol is less pronounced than that of a previous investigation using Tween 80, indicating its potential as the more favourable choice for culturing M. tuberculosis for metabolomic analysis, with due consideration to dosage and result interpretation.

泰乐菌素对结核分枝杆菌代谢组的影响
在培养结核杆菌时,使用洗涤剂对防止结块至关重要。然而,这些洗涤剂可能会影响细菌的形态和新陈代谢,从而影响研究结果。本研究旨在评估使用泰乐菌素培养的结核杆菌H37Rv菌株(H37RvTyloxapol)与不使用洗涤剂培养的H37Rv菌株对照组(H37RvControl)的代谢组,以评估泰乐菌素是否适合代谢组学研究。与 H37RvControl 相比,在 H37RvTyloxapol 中观察到了不同的代谢变化,主要与脂肪酸、糖和磷酸戊糖代谢途径有关。这些变化与泰乐菌素对细菌造成的表面压力有关,促使结核杆菌的新陈代谢适应通常在压力环境下观察到的新陈代谢。尽管如此,与之前使用吐温 80 进行的调查相比,tyloxapol 的影响并不那么明显,这表明在适当考虑用量和结果解释的情况下,tyloxapol 有可能成为培养结核杆菌进行代谢组学分析的更有利选择。
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来源期刊
Journal of microbiological methods
Journal of microbiological methods 生物-生化研究方法
CiteScore
4.30
自引率
4.50%
发文量
151
审稿时长
29 days
期刊介绍: The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.
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