Immunophenotypic and molecular changes during progression of papillary urothelial carcinoma.

IF 2.5 3区 医学 Q2 UROLOGY & NEPHROLOGY
Bohyun Kim, Kwangsoo Kim, Sunah Yang, Kyung Chul Moon
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引用次数: 0

Abstract

Purpose: Urothelial carcinoma has various molecular subtypes, each with different tumor characteristics. Although it is known that molecular changes occur during tumor progression, little is known about the specifics of these changes. In this study, we performed transcriptional analysis to understand the molecular changes during tumor progression.

Materials and methods: Formalin-fixed, paraffin-embedded tumor tissues were obtained from 12 patients with muscle-invasive bladder cancer (MIBC). The invasive and non-invasive papillary areas were identified in papillary urothelial carcinoma specimens. Immunohistochemistry (IHC) and mRNA sequencing were performed for each tumor area.

Results: Patients with CK5/6-negative and CK20-positive non-invasive papillary areas were selected and classified into the IHC switch subgroup (CK5/6-positive and CK20-negative in the invasive area) and the IHC unchanged subgroup (CK5/6-negative and CK20-positive in the invasive area) according to the IHC results of the invasive area. We identified differences in the mRNA expression between the non-invasive papillary and invasive areas of the papillary MIBC tissue samples. In both the non-invasive papillary and invasive areas, the IHC switch subgroup showed basal subtype gene expression, while the IHC unchanged subgroup demonstrated luminal subtype gene expression.

Conclusions: The non-invasive papillary area showed a gene expression pattern similar to that of the invasive area. Therefore, even if the non-invasive papillary area exhibits a luminal phenotype on IHC, it can have a basal subtype gene expression depending on the invasive area.

乳头状尿路上皮癌发展过程中的免疫表型和分子变化。
目的:尿路上皮癌有多种分子亚型,每种亚型都有不同的肿瘤特征。虽然人们知道肿瘤进展过程中会发生分子变化,但对这些变化的具体情况却知之甚少。在本研究中,我们进行了转录分析,以了解肿瘤进展过程中的分子变化:12例肌浸润性膀胱癌(MIBC)患者的福尔马林固定、石蜡包埋的肿瘤组织。在乳头状尿路上皮癌标本中确定了浸润性和非浸润性乳头区。对每个肿瘤区域进行免疫组化(IHC)和 mRNA 测序:根据浸润区的 IHC 结果,选择了 CK5/6 阴性和 CK20 阳性的非浸润性乳头区患者,并将其分为 IHC 切换亚组(浸润区 CK5/6 阳性和 CK20 阴性)和 IHC 不变亚组(浸润区 CK5/6 阴性和 CK20 阳性)。我们发现乳头状 MIBC 组织样本的非浸润性乳头区和浸润性区的 mRNA 表达存在差异。在非浸润性乳头状区域和浸润性区域,IHC转换亚组显示基底亚型基因表达,而IHC不变亚组显示管腔亚型基因表达:结论:非浸润性乳头区的基因表达模式与浸润区相似。结论:非浸润性乳头区的基因表达模式与浸润区相似,因此,即使非浸润性乳头区在 IHC 上表现为管腔表型,它也可以根据浸润区的情况表现为基底亚型基因表达。
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来源期刊
CiteScore
4.10
自引率
4.30%
发文量
82
审稿时长
4 weeks
期刊介绍: Investigative and Clinical Urology (Investig Clin Urol, ICUrology) is an international, peer-reviewed, platinum open access journal published bimonthly. ICUrology aims to provide outstanding scientific and clinical research articles, that will advance knowledge and understanding of urological diseases and current therapeutic treatments. ICUrology publishes Original Articles, Rapid Communications, Review Articles, Special Articles, Innovations in Urology, Editorials, and Letters to the Editor, with a focus on the following areas of expertise: • Precision Medicine in Urology • Urological Oncology • Robotics/Laparoscopy • Endourology/Urolithiasis • Lower Urinary Tract Dysfunction • Female Urology • Sexual Dysfunction/Infertility • Infection/Inflammation • Reconstruction/Transplantation • Geriatric Urology • Pediatric Urology • Basic/Translational Research One of the notable features of ICUrology is the application of multimedia platforms facilitating easy-to-access online video clips of newly developed surgical techniques from the journal''s website, by a QR (quick response) code located in the article, or via YouTube. ICUrology provides current and highly relevant knowledge to a broad audience at the cutting edge of urological research and clinical practice.
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