PIN1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB.

IF 3.5 4区 生物学 Q1 Biochemistry, Genetics and Molecular Biology
Sinem Saritas Erdogan, Ahmet Erdal Yilmaz, Asli Kumbasar
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引用次数: 0

Abstract

NFIB is a transcription factor of the Nuclear Factor One (NFI) family that is essential for embryonic development. Post-translational control of NFIB or its upstream regulators have not been well characterized. Here, we show that PIN1 binds NFIB in a phosphorylation-dependent manner, via its WW domain. PIN1 interacts with the well-conserved N-terminal domains of all NFIs. Moreover, PIN1 attenuates the transcriptional activity of NFIB; this attenuation requires substrate binding by PIN1 but not its isomerase activity. Paradoxically, we found stabilization of NFIB by PIN1. We propose that PIN1 represses NFIB function not by regulating its abundance but by inducing a conformational change. These results identify NFIB as a novel PIN1 target and posit a role for PIN1 in post-translational regulation of NFIB and other NFIs.

PIN1 是转录因子 NFIB 的新型相互作用伙伴和上游负调控因子。
NFIB 是核因子一(NFI)家族的转录因子,对胚胎发育至关重要。NFIB 或其上游调节因子的翻译后控制尚未得到很好的描述。在这里,我们发现 PIN1 通过其 WW 结构域以磷酸化依赖的方式与 NFIB 结合。PIN1 与所有 NFIs 的保守的 N 端结构域相互作用。此外,PIN1 还能减弱 NFIB 的转录活性;这种减弱需要 PIN1 与底物结合,但不需要其异构酶活性。矛盾的是,我们发现 PIN1 能稳定 NFIB。我们认为,PIN1 不是通过调节 NFIB 的丰度,而是通过诱导构象变化来抑制 NFIB 的功能。这些结果确定了 NFIB 是 PIN1 的新靶标,并认为 PIN1 在 NFIB 和其他 NFIs 的翻译后调控中发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
7.00
自引率
2.90%
发文量
303
审稿时长
1.0 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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