An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro.

IF 2.5 4区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Yuanyong Yao, Tao Wu, Meng Zhang, Daihua Fu, Hai Yang, Shixue Chen
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Abstract

The difference on inhibitory effects of bioflavonoids inhibiting XOD activity assayed by varying test methods cause of us to be further in consideration. The reported test method creating a micro-environment surrounding XOD in the absence of ⋅O2 -, which is seemly different from the assay in vivo. So, the vitro test method for assaying XOD activity is necessary to be improved for selection of potential inhibitors in the presence of ⋅O2 -. The inhibitory results demonstrated that bioflavonoids of MY, DMY, QUE and LUT are capable to be on effective IC50 values, but others are not. As well, their resulting inhibitions determined by the improved test method are much less than that reported in the literature, indicating that their chemical affinities with XOD become weaker. Moreover, DMY assayed on the inhibitions of XOD in the improved test method performs to be a better inhibitor, as compared to the assay of the reported test methods. Abasing on the transformation of DMY into MY in the presence of ⋅O2 -, the good inhibition of DMY on XOD activity can be explained by the synergistic effect of MY.

Abstract Image

体外测定生物类黄酮对黄嘌呤氧化酶活性抑制的改进测试法
不同试验方法测定的生物类黄酮对 XOD 活性的抑制作用存在差异,这引起了我们的进一步思考。所报道的测试方法是在没有 ⋅O2 的情况下在 XOD 周围创造一个微环境,这似乎与体内的测试方法不同。因此,有必要改进用于检测 XOD 活性的体外测试方法,以筛选出存在 ⋅O2 - 的潜在抑制剂。抑制结果表明,MY、DMY、QUE 和 LUT 的生物类黄酮能够达到有效的 IC50 值,而其他生物类黄酮则不能。此外,用改进的测试方法测定的它们的抑制作用也比文献报道的要小得多,这表明它们与 XOD 的化学亲和力变弱了。此外,与已报道的测试方法相比,改进测试方法中对 XOD 抑制作用进行测定的 DMY 是一种更好的抑制剂。根据 DMY 在 ⋅O2 - 的存在下转化为 MY 的情况,DMY 对 XOD 活性的良好抑制作用可以解释为 MY 的协同作用。
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来源期刊
ChemistryOpen
ChemistryOpen CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
4.80
自引率
4.30%
发文量
143
审稿时长
1 months
期刊介绍: ChemistryOpen is a multidisciplinary, gold-road open-access, international forum for the publication of outstanding Reviews, Full Papers, and Communications from all areas of chemistry and related fields. It is co-owned by 16 continental European Chemical Societies, who have banded together in the alliance called ChemPubSoc Europe for the purpose of publishing high-quality journals in the field of chemistry and its border disciplines. As some of the governments of the countries represented in ChemPubSoc Europe have strongly recommended that the research conducted with their funding is freely accessible for all readers (Open Access), ChemPubSoc Europe was concerned that no journal for which the ethical standards were monitored by a chemical society was available for such papers. ChemistryOpen fills this gap.
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