Antimicrobial and Cytotoxic Effect of Bornetella Nitida Green Algae Isolate on MCF-7 Breast Cancer.

IF 3.3 4区医学 Q2 ONCOLOGY

Breast Cancer : Targets and Therapy Pub Date : 2024-09-04 eCollection Date: 2024-01-01 DOI:10.2147/BCTT.S469036

Yuni Elsa Hadisaputri, Nunuk Hariani Soekamto, Pratiwi Pudjiastuti, Aria Aristokrat, Bahrun Bahrun, Fajar Fauzi Abdullah, Tatsufumi Okino

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Abstract

Introduction: Marine algae are increasingly becoming a potential resource for new drugs. In recent decades, including Bornetella nitida (B. nitida). Meanwhile, antimicrobial and anticancer agents are the first line of choice for developing alternative compounds, considering the annually increasing resistant events. Therefore, this study aimed to examine the antimicrobial and cytotoxic potential of B. nitida isolate compounds.

Methods: The B. nitida resulted in 2 compounds, sitosterol 3β tetracosanoate and (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol. Both compounds were tested to have antibacterial effects against Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Methicillin-resistant Staphylococcus Aureus (MRSA). Proliferation assay was conducted using the PrestoBlue™ Cell Viability Reagent, which was also used to measure the IC₅₀ against MCF-7 breast cancer cells.

Results: The results showed that (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol had antimicrobial activity against Staphylococcus aureus and IC₅₀ value of 142.18 µg/mL against MCF-7 cells, while sitosterol 3β tetracosanoate does not have any antimicrobial activity and IC₅₀ value of 681.65 µg/mL. Moreover, the mechanism prediction using docking with caspase-3 receptor to induce apoptosis was also evaluated.

Conclusion: Based on the results, (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol of B. nitida has great potential as an antimicrobial and anticancer agent.

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Bornetella Nitida 绿藻隔离物对 MCF-7 乳腺癌的抗菌和细胞毒性作用

导言：海洋藻类正日益成为新药的潜在资源。近几十年来，包括 Bornetella nitida（B. nitida）在内的海洋藻类已成为新药开发的潜在资源。同时，考虑到逐年增加的抗药性事件，抗菌剂和抗癌剂是开发替代化合物的第一线选择。因此，本研究旨在考察硝化杆菌分离物化合物的抗菌和细胞毒性潜力：硝化纤维芽孢杆菌产生了两种化合物：3β-十四烷酸 sitosterol 和(E)-17-(8-乙基-4,5,9-三甲基癸-6-烯-2-基)-13-甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢-1H-环戊并[a]菲-3-醇。这两种化合物对绿脓杆菌、枯草杆菌、金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）均有抗菌作用。使用 PrestoBlue™ 细胞活力试剂进行了增殖试验，并测定了对 MCF-7 乳腺癌细胞的 IC50：结果表明，(E)-17-(8-乙基-4,5,9-三甲基癸-6-烯-2-基)-13-甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢-1H-环戊并[a]菲-3-醇对金黄色葡萄球菌具有抗菌活性，对 MCF-7 乳腺癌细胞的 IC50 值为 142.18微克/毫升，而3β-十四烷酸西甾醇酯没有任何抗菌活性，IC50值为681.65微克/毫升。此外，还评估了与 caspase-3 受体对接诱导细胞凋亡的机制预测：根据研究结果，硝化纤维芽孢杆菌的(E)-17-(8-乙基-4,5,9-三甲基癸-6-烯-2-基)-13-甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢-1H-环戊并[a]菲-3-醇具有作为抗菌剂和抗癌剂的巨大潜力。

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