A flexible strategy to fabricate trumpet-shaped porous PDMS membranes for organ-on-chip application.

IF 2.6 4区 工程技术 Q2 BIOCHEMICAL RESEARCH METHODS
Biomicrofluidics Pub Date : 2024-09-05 eCollection Date: 2024-09-01 DOI:10.1063/5.0227148
Yingying Xie, Yaqiong Guo, Fuwei Xie, Yan Dong, Xiaoqing Zhang, Xiang Li, Xu Zhang
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引用次数: 0

Abstract

Porous polydimethylsiloxane (PDMS) membrane is a crucial element in organs-on-chips fabrication, supplying a unique substrate that can be used for the generation of tissue-tissue interfaces, separate co-culture, biomimetic stretch application, etc. However, the existing methods of through-hole PDMS membrane production are largely limited by labor-consuming processes and/or expensive equipment. Here, we propose an accessible and low-cost strategy to fabricate through-hole PDMS membranes with good controllability, which is performed via combining wet-etching and spin-coating processes. The porous membrane is obtained by spin-coating OS-20 diluted PDMS on an etched glass template with a columnar array structure. The pore size and thickness of the PDMS membrane can be adjusted flexibly via optimizing the template structure and spinning speed. In particular, compared to the traditional vertical through-hole structure of porous membranes, the membranes prepared by this method feature a trumpet-shaped structure, which allows for the generation of some unique bionic structures on organs-on-chips. When the trumpet-shape faces upward, the endothelium spreads at the bottom of the porous membrane, and intestinal cells form a villous structure, achieving the same effect as traditional methods. Conversely, when the trumpet-shape faces downward, intestinal cells spontaneously form a crypt-like structure, which is challenging to achieve with other methods. The proposed approach is simple, flexible with good reproducibility, and low-cost, which provides a new way to facilitate the building of multifunctional organ-on-chip systems and accelerate their translational applications.

为芯片上器官应用制造喇叭形多孔 PDMS 膜的灵活策略。
多孔聚二甲基硅氧烷(PDMS)膜是芯片上器官制造的关键要素,它提供了一种独特的基底,可用于生成组织-组织界面、分离共培养、生物仿生拉伸应用等。然而,现有的通孔 PDMS 膜生产方法在很大程度上受到耗费人力的工艺和/或昂贵设备的限制。在此,我们提出了一种简便易行且成本低廉的策略,通过结合湿法蚀刻和旋涂工艺来制造具有良好可控性的通孔 PDMS 膜。多孔膜是通过在具有柱状阵列结构的蚀刻玻璃模板上旋涂 OS-20 稀释 PDMS 而获得的。通过优化模板结构和旋涂速度,可以灵活调整 PDMS 膜的孔径和厚度。特别值得一提的是,与传统多孔膜的垂直通孔结构相比,该方法制备的膜具有喇叭形结构,可在芯片器官上生成一些独特的仿生结构。当喇叭形朝上时,内皮细胞在多孔膜底部扩散,肠细胞形成绒毛状结构,达到与传统方法相同的效果。相反,当喇叭形朝下时,肠细胞会自发形成隐窝状结构,这是其他方法难以实现的。所提出的方法简单、灵活、重现性好、成本低,为促进多功能片上器官系统的构建和加速其转化应用提供了一条新途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomicrofluidics
Biomicrofluidics 生物-纳米科技
CiteScore
5.80
自引率
3.10%
发文量
68
审稿时长
1.3 months
期刊介绍: Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics. Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary) Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification) Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation) Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles) Cell culture and analysis(single cell assays, stimuli response, stem cell transfection) Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays) Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers) Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...
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