Characterization of cyclotides Mra30 and cycloviolacin O17 derived from Viola dalatensis Gadnep

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Tien T. Dang, Tam T. T. Tran, Son H. Pham, Tong-Hung Quach, Nhu T.Q. Ngo, Tuan H.N. Nguyen
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引用次数: 0

Abstract

Bacteria threaten human and animal health, and standard antibiotics no longer effective. Antibiotic-resistant microorganisms can make infection treatment challenging and perhaps fail. Investigating the attributes of cyclotide, a peptide with promising antibacterial properties that holds great potential in the field of antibiotic research. The structure of these cyclic peptides involves six conserved cysteine residues that form three disulfide bonds, resulting in a cyclic cystine knot (CCK). This feature guarantees their durability when exposed to changes in temperature, chemicals, and enzymatic degradation. The two cyclotides, cycloviolacin O17 and mra30, were obtained from Viola dalatensis Gadnep through a series of techniques including the use of a 50% acetonitrile/49% miliQ water/1% formic acid solution for extraction, ammonium salt precipitation, RP-HPLC purification and sequence identification by LC-MS/MS. These cyclotides exhibit antibacterial effects on specific strains of bacteria like Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa at a concentration of 0.2 mg/mL, leading to inhibition zones ranging from 10 to 14 mm. In addition, the disulfide bonds play a crucial role in the antibacterial function of cyclotides. Disrupting the disulfide bonds through ankylation reaction results in the loss of antibacterial properties in the cyclotides (cyO17 and mra30). The minimum inhibitory concentration (MIC) values of mra30 and cyO17 are significantly low, ranging from 0.1 to 0.6 µM. These values are approximately three times lower than the MIC values observed in salt precipitation samples.

Abstract Image

从 Viola dalatensis Gadnep 中提取的环素 Mra30 和环紫杉素 O17 的特征。
细菌威胁着人类和动物的健康,而标准抗生素已不再有效。抗生素耐药微生物会使感染治疗面临挑战,甚至失败。环肽是一种具有良好抗菌特性的多肽,在抗生素研究领域具有巨大潜力。这些环肽的结构包括六个保守的半胱氨酸残基,它们形成三个二硫键,形成环状胱氨酸结(CCK)。这一特点保证了它们在温度变化、化学物质和酶降解时的耐久性。通过一系列技术,包括使用 50% 乙腈/49% miliQ 水/1% 甲酸溶液提取、铵盐沉淀、RP-HPLC 纯化和 LC-MS/MS 序列鉴定,从 Viola dalatensis Gadnep 中获得了两种环苷酸:环紫草素 O17 和 mra30。在 0.2 毫克/毫升的浓度下,这些环苷酸对金黄色葡萄球菌、枯草杆菌和铜绿假单胞菌等特定菌株具有抗菌作用,抑制区范围为 10 至 14 毫米。此外,二硫键在环苷酸的抗菌功能中起着至关重要的作用。通过烷基化反应破坏二硫键会导致环苷酸(cyO17 和 mra30)失去抗菌特性。mra30 和 cyO17 的最低抑菌浓度 (MIC) 值很低,在 0.1 至 0.6 µM 之间。这些值比在盐沉淀样品中观察到的 MIC 值低约三倍。
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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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