Expression of cancer susceptibility candidate 11 in ovarian cancer tissues and its role in doxorubicin resistance.

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2024-12-01 Epub Date: 2024-09-09 DOI:10.1007/s10735-024-10254-w

Kui Yao, Heng Zheng, Longxia Tong

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Abstract

We aimed to investigate the expression of cancer susceptibility candidate 11 (CASC11) in ovarian cancer (OC) tissues and its role in doxorubicin (Dox) resistance. A total of 98 patients were included as subjects. Reverse transcription-polymerase chain reaction was employed to determine the expressions of CASC11 in OC and para-OC tissues, and in OC cells (A2780, SKOV3, OVCAR3 and A547) and human normal ovarian epithelial cells (IOSE-80) from these patients. OC SKOV3/R cell line with Dox resistance was established and transfected with small interfering (si)-CASC11 to down-regulate CASC11 expression. Based on the constructed nude mouse model of orthotopic transplanted tumor, the growth curves were plotted, and the changes in tumor volume and apoptosis were observed by hematoxylin-eosin staining. OC tissues had a significantly higher mRNA expression of CASC11 than that of para-OC tissues (P < 0.05). A547, OVCAR3, A2780 and SKOV3 cells had significantly higher mRNA expressions of CASC11 than that of IOSE-80 cells (P < 0.05). The transplanted tumor was significantly smaller in volume in the si-CASC11 group than that in the si-normal control (NC) group from the 8th days after transplanted tumor inoculation (P < 0.05). The tumor growth inhibition rate significantly rose in the si-CASC11 group in comparison with that in the si-NC group (P < 0.05). CASC11 has high expression in OC tissues. Knockout of CASC11 weakens the proliferative, invasive and migratory potentials and enhances the apoptotic potential of Dox-resistant OC cells, thereby reversing their Dox resistance.

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癌症易感性候选基因 11 在卵巢癌组织中的表达及其在多柔比星耐药性中的作用。

我们旨在研究癌症易感性候选基因 11（CASC11）在卵巢癌（OC）组织中的表达及其在多柔比星（Dox）耐药性中的作用。研究对象包括98名患者。研究人员采用反转录聚合酶链反应检测了 CASC11 在卵巢癌和副卵巢癌组织中的表达，以及在这些患者的卵巢癌细胞（A2780、SKOV3、OVCAR3 和 A547）和人类正常卵巢上皮细胞（IOSE-80）中的表达。建立了具有Dox耐药性的OC SKOV3/R细胞系，并用小干扰（si）-CASC11转染以下调CASC11的表达。在构建的裸鼠正位移植肿瘤模型基础上，绘制肿瘤生长曲线，并通过苏木精-伊红染色观察肿瘤体积和凋亡的变化。OC组织的CASC11 mRNA表达量明显高于副OC组织（P＜0.05）。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.