Rapid miRNA detection in skin interstitial fluid using a hydrogel microneedle patch integrated with DNA probes and graphene oxide†

IF 6.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Lab on a Chip Pub Date : 2024-09-06 DOI:10.1039/D4LC00715H

Hanjia Zheng, Fatemeh Keyvani, Sadegh Sadeghzadeh, Dragos F. Mantaila, Fasih A. Rahman, Joe Quadrilatero and Mahla Poudineh

{"title":"Rapid miRNA detection in skin interstitial fluid using a hydrogel microneedle patch integrated with DNA probes and graphene oxide†","authors":"Hanjia Zheng, Fatemeh Keyvani, Sadegh Sadeghzadeh, Dragos F. Mantaila, Fasih A. Rahman, Joe Quadrilatero and Mahla Poudineh","doi":"10.1039/D4LC00715H","DOIUrl":null,"url":null,"abstract":"<p >MicroRNA (miRNA) is a type of short, non-coding nucleic acid molecule that plays essential roles in diagnosing and prognosing various types of cancer. MiRNA is abundantly present in skin interstitial fluid (ISF), providing real-time and localized physiological information. Hydrogel microneedle (HMN) patches enable miRNA collection in a fast, pain-free, minimally invasive, and user-friendly manner. In this study, we introduced a fluorescence-based HMN assay, namely the HMN-miR sensor, composed of methacrylated hyaluronic acid (MeHA) and a graphene oxide–probe DNA (GO.pDNA) conjugate for miR21 and miR210 detection. The HMN-miR sensor demonstrates excellent skin penetration efficiency, rapid ISF collection capability, and sufficient miRNA detection and sequence identification specificity. The HMN-miR sensor facilitates a new assay that, with further optimization, could be applied in future clinical settings. Its simple fabrication process and excellent biocompatibility give it significant potential for various clinical uses, such as personalized cancer treatment and monitoring the healing progress of burn wounds.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 4989-4997"},"PeriodicalIF":6.1000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00715h","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

MicroRNA (miRNA) is a type of short, non-coding nucleic acid molecule that plays essential roles in diagnosing and prognosing various types of cancer. MiRNA is abundantly present in skin interstitial fluid (ISF), providing real-time and localized physiological information. Hydrogel microneedle (HMN) patches enable miRNA collection in a fast, pain-free, minimally invasive, and user-friendly manner. In this study, we introduced a fluorescence-based HMN assay, namely the HMN-miR sensor, composed of methacrylated hyaluronic acid (MeHA) and a graphene oxide–probe DNA (GO.pDNA) conjugate for miR21 and miR210 detection. The HMN-miR sensor demonstrates excellent skin penetration efficiency, rapid ISF collection capability, and sufficient miRNA detection and sequence identification specificity. The HMN-miR sensor facilitates a new assay that, with further optimization, could be applied in future clinical settings. Its simple fabrication process and excellent biocompatibility give it significant potential for various clinical uses, such as personalized cancer treatment and monitoring the healing progress of burn wounds.

Abstract Image

查看原文本刊更多论文

利用集成 DNA 探针和氧化石墨烯的水凝胶微针贴片从皮肤间质液中快速检测 miRNA 的潜在测定方法

微小核糖核酸（miRNA）是一种短小的非编码核酸分子，在各种癌症的诊断和预后中发挥着重要作用。MiRNA 大量存在于皮肤间质（ISF）中，可提供实时和局部的生理信息。水凝胶微针（HMN）贴片能以快速、无痛、微创和用户友好的方式收集 miRNA。在这项研究中，我们引入了一种基于荧光的 HMN 检测方法，命名为 HMN-miR 传感器，由甲基丙烯酸化透明质酸（MeHA）和氧化石墨烯-探针 DNA（GO.pDNA）共轭物组成，用于检测 miR21 和 miR210。HMN-miR 传感器具有出色的皮肤穿透效率、快速的 ISF 采集能力以及足够的 miRNA 检测和序列鉴定特异性。HMN-miR 传感器引入了一种新的检测方法，经过进一步优化，可应用于未来的临床环境。其简单的制造过程和出色的生物相容性使其在各种临床应用中具有巨大潜力，如个性化癌症治疗和烧伤伤口愈合进展监测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Lab on a Chip 工程技术-化学综合

CiteScore

11.10

自引率

8.20%

发文量

434

审稿时长

2.6 months

期刊介绍： Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.