Polar-localized OsLTPG22 regulates rice leaf cuticle deposition and drought response

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2024-09-07 DOI:10.1016/j.stress.2024.100586

Zhongyuan Chang , Minzhang Zhao , Baoxiang Qin , Lilan Hong

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引用次数: 0

Abstract

The cuticle serves as a crucial protective barrier for plant survival, and recent studies have highlighted the essential roles of nonspecific lipid transfer proteins (nsLTPs) in cuticle formation. However, the specific function of nsLTPs in the rice leaf cuticle remains unclear. In this study, we functionally characterized OsLTPG22, a G-type nsLTP with a signal peptide (SP) domain and a glycosylphosphatidylinositol (GPI) anchor region. Mutation in OsLTPG22 led to a reduction in cuticular wax abundance, increased leaf epidermal permeability, and higher drought sensitivity in seedlings. OsLTPG22 was widely expressed in various tissues and exhibited distinct polar localization to the aerial surface of epidermal cells in expanding leaves. OsLTPG22 binds lipids and localizes to the plasma membrane. Protein truncation experiments demonstrated that OsLTPG22’s polar localization was regulated by the SP domain, while both the SP domain and GPI anchor region regulated OsLTPG22’s plasma membrane localization. This work provides genetic and cytological evidence for OsLTPG22’s role in leaf cuticle formation and drought response, enhancing our understanding of nsLTP function and offering insights for breeding drought-resistant crops.

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极定位的 OsLTPG22 调控水稻叶片角质层沉积和干旱响应

角质层是植物生存的重要保护屏障，最近的研究强调了非特异性脂质转移蛋白（nsLTPs）在角质层形成过程中的重要作用。然而，nsLTPs 在水稻叶片角质层中的具体功能仍不清楚。在这项研究中，我们对 OsLTPG22 进行了功能鉴定，它是一种具有信号肽（SP）结构域和糖基磷脂酰肌醇（GPI）锚区的 G 型 nsLTP。OsLTPG22 的突变导致幼苗的角质蜡丰度降低、叶表皮渗透性增加以及对干旱的敏感性提高。OsLTPG22 在各种组织中广泛表达，并在膨大叶片表皮细胞的气生表面表现出明显的极性定位。OsLTPG22 与脂质结合并定位在质膜上。蛋白截短实验表明，OsLTPG22的极性定位受SP结构域的调控，而SP结构域和GPI锚区都调控OsLTPG22的质膜定位。这项工作为 OsLTPG22 在叶片角质层形成和干旱响应中的作用提供了遗传学和细胞学证据，加深了我们对 nsLTP 功能的理解，并为培育抗旱作物提供了启示。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.