The down-regulation of salivary protein gene expression by etofenprox partially contributed to reducing the risk of increased fecundity in the brown planthopper

IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
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Abstract

Etofenprox is a pyrethroid insecticide that acts on the nervous system of insects. Due to its low toxicity to aquatic animals, it is permitted for use in controlling insect pests in rice fields. The brown planthopper (BPH), Nilaparvata lugens, a significant piercing-sucking pest feeding on rice exclusively, secretes various salivary components when feeding. Salivary proteins are essential for BPH feeding, but their response to etofenprox is not well understood. The application of etofenprox down-regulated the expression of 21 salivary protein genes, among which 9 genes (NlShpa, Salivap 3, CA, NlSEF1, Nl12, NlHSC70–3, NlSP1, NlG14, and NlDNAJB9) showed significant differences. Most differentially expressed genes are found important for BPH physiological processes, except Nl12. Here we found that silencing Nl12 impeded ovary development, thereby inhibiting oocyte formation. The potential explanation was that Nl12 was highly expressed in both salivary gland and ovary, and the ovary development abnormality may be due to the direct effect from expression reduction in ovary and/or indirect influence from expression reduction in salivary gland. Altogether, our findings provide a new insight into the mechanism of action of etofenprox on insect pests and explain part of the reason why etofenprox does not stimulate reproduction in BPH.

Abstract Image

醚菊酯对唾液蛋白基因表达的下调在一定程度上有助于降低褐飞虱繁殖力增加的风险
Etofenprox 是一种拟除虫菊酯杀虫剂,作用于昆虫的神经系统。由于它对水生动物的毒性较低,因此被允许用于控制稻田害虫。褐飞虱 Nilaparvata lugens 是一种专门以水稻为食的重要穿孔吸食害虫,在取食时会分泌多种唾液成分。唾液蛋白对 BPH 的取食至关重要,但它们对醚菊酯的反应却不甚了解。施用醚菊酯会下调 21 个唾液蛋白基因的表达,其中 9 个基因(NlShpa、Salivap 3、CA、NlSEF1、Nl12、NlHSC70-3、NlSP1、NlG14 和 NlDNAJB9)的表达有显著差异。除 Nl12 外,大多数差异表达基因对良性前列腺增生症的生理过程非常重要。在这里,我们发现沉默 Nl12 会阻碍卵巢发育,从而抑制卵母细胞的形成。可能的解释是,Nl12在唾液腺和卵巢中均高表达,卵巢发育异常可能是由于卵巢中表达减少的直接影响和/或唾液腺中表达减少的间接影响。总之,我们的研究结果为了解醚菊酯对害虫的作用机制提供了新的视角,并解释了醚菊酯不能刺激鳞翅目害虫繁殖的部分原因。
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来源期刊
CiteScore
7.00
自引率
8.50%
发文量
238
审稿时长
4.2 months
期刊介绍: Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.
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